The inhibitory effect of artesunate on hepatocellular carcinoma cells by regulating expression of GADD45A and NACC1
- VernacularTitle:青蒿琥酯通过调控GADD45A、NACC1的表达抑制肝癌细胞的作用
- Author:
Guan-Tong SHEN
1
;
Jin-Yao DONG
;
Jing FENG
;
Nan QIN
;
Gen-Lai DU
;
Fei ZHU
;
Ke LIAN
;
Xin-Yu LIU
;
Qing-Liang LI
;
Xun-Wei ZHANG
;
Ru-Yi SHI
Author Information
- Keywords: artesunate; hepatocellular carcinoma; an-ticancer mechanism; GADD45A; NACC1; apoptosis; G2/M
- From: Chinese Pharmacological Bulletin 2024;40(6):1089-1097
- CountryChina
- Language:Chinese
- Abstract: Aim To explore the effect and mechanism of the artesunate(ART)on hepatocellular carcinoma(HCC).Methods The cell lines MHCC-97H and HCC-LM3 were used to be detected.MTT and clone formation were used to determine the cell proliferation;Wound healing was used to detect the cell migration;Transwell was used to test the cell invasion.Flow-cy-tometry was used to detect cell apoptosis and cell cy-cle.RNA-seq and qRT-PCR was used to detect the genes expression.Results The proliferation,migra-tion and invasion of treated cells were obviously inhibi-ted(P＜0.01).Moreover,the apoptosis rate in-creased significantly,so did the proportion of G2/M cells.Transcriptomic analysis identified GADD45A as a potential target of ART through RNA-sequencing da-ta,and suggested that ART might induce apoptosis and cell cycle arrest through regulating the expression of GADD45A.In addition,the results of mechanism studies and signaling analysis suggested that GADD45A had interaction with its upstream gene NACC1(nucle-us accumbens associated 1).Moreover,after ART treatment,the expressions of GADD45A and NACC1 were changed significantly.Conclusion ART may be a potential drug to resist HCC by affecting the expres-sion of GADD45A and its upstream gene NACC1,which provides a new drug,a new direction and a new method for the clinical treatment of HCC.
