LncRNA UNC5B-AS1 regulates malignant biological behavior of osteosarcoma cells through NF-κB signaling pathways
10.12360/CPB202401063
- VernacularTitle:lncRNA UNC5B-AS1通过NF-κB信号通路调控骨肉瘤细胞的恶性生物学行为
- Author:
Qing-Lin YANG
1
;
Huai-Bin ZHANG
;
Zhi-Jie LAN
;
Qing-Qing QIN
;
Yi-Kun WANG
;
Yong-Ping WANG
Author Information
1. 兰州大学第一临床医学院
- Keywords:
osteosarcoma;
lncRNA UNC5B-AS1;
NF-κB signaling pathway;
proliferation;
apoptosis;
mechanism
- From:
Chinese Pharmacological Bulletin
2024;40(6):1082-1088
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the possible mecha-nism of UNC5B-AS1 in regulating the malignant biolog-ical behavior of osteosarcoma cells.Methods RT-qPCR was used to detect the expression of UNC5B-AS1 in osteosarcoma cells MG63,osteosarcoma cells U2OS and osteoblast cells hFOB1.19.After overexpression and knockdown of UNC5B-AS1 in osteosarcoma cells,the proliferation,migration and apoptosis of osteosarco-ma cells were detected by CCK-8 assay,Transwell as-say and flow cytometry,respectively.At the same time,RT-qPCR and Western blot were used to detect the effects of UNC5B-AS1 overexpression and knock-down on the mRNA and protein expression of key fac-tors in the NF-κB signaling pathway.Results Com-pared with normal osteoblast hFOB1.19,UNC5B-AS1 expression was differentially increased in osteosarcoma cells MG63 and U2OS.Overexpression of UNC5B-AS1 significantly promoted the proliferation of osteosarcoma cells and significantly increased the migration ability of osteosarcoma cells,while the apoptosis rate markedly decreased,and NF-κB signaling pathway-related mR-NA and protein expressions apparently increased.Knockdown of UNC5B-AS1 evidently inhibited the pro-liferation of osteosarcoma cells and significantly re-duced the migration ability of osteosarcoma cells,while the apoptosis rate markedly increased,the NF-κB sig-naling pathway related mRNA and protein expression significantly reduced.Conclusions lncRNA UNC5B-AS1 is highly expressed in osteosarcoma cells,which may affect the malignant biological behavior of osteo-sarcoma cells by activating the NF-κB signaling path-way.
