Effects of Tao Hong Si Wu decoction on circular RNA expression profiles in rats with middle cerebral artery occlusion
- VernacularTitle:桃红四物汤对大脑中动脉闭塞大鼠环状RNA表达谱的影响
- Author:
Chang-Yi FEI
1
,
2
;
Li-Juan ZHANG
;
Ni WANG
;
Fu-Rui CHU
;
Chao YU
;
Su-Jun XUE
;
Ling-Yu PAN
;
Dai-Yin PENG
;
Xian-Chun DUAN
Author Information
- Keywords: Tao Hong Si Wu decoction; middle cere-bral artery occlusion; circRNA; bioinformatics; next-generation RNA sequencing; ischemic stroke
- From: Chinese Pharmacological Bulletin 2024;40(5):954-963
- CountryChina
- Language:Chinese
- Abstract: Aim To screen and study the effects of Tao Hong Si Wu decoction(THSWD)-mediated treat-ment on circular RNA(circRNA)expression profiles in rats with middle cerebral artery occlusion(MCAO),and investigate the possible roles and molecular mecha-nisms of THSWD.Methods Next-generation RNA sequencing was conducted to identify circRNA expres-sion profiles in MCAO rats after treatment with THSWD and compared with the MCAO model group and control group.Bioinformatics analysis was performed to predict the potential target microRNAs and mRNAs.Gene On-tology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway analyses for the potential target mRNAs were applied to explore the potential roles of differentially expressed circRNAs.RT-qPCR was performed to verify circRNAs with significant differences in expression.Results We identified 87 significantly differentially expressed circRNAs between the MCAO group versus the control group,and 86 sig-nificantly differentially expressed circRNAs between the MCAO group versus the THSWD group.respective-ly.Among them,17 circRNAs induced by the MCAO model were reversed via treatment with THSWD.To demonstrate the roles of mRNAs targeted by DECs,the GO and KEGG databases were used.Further analysis revealed that five circRNAs may play important roles in the development of MCAO.Conclusions The com-prehensive expression profile of circRNAs in rats with middle cerebral artery occlusion after THSWD treat-ment is determined for the first time,suggesting that the therapeutic effect of THSWD on MCAO may be a-chieved by regulating the expression of circRNAs.
