Effect of knockdown of ARHGAP30 on proliferation and apoptosis of Siha cells
- VernacularTitle:敲减ARHGAP30基因对宫颈癌Siha细胞增殖及凋亡的影响
- Author:
Ya-Ting PENG
1
;
Duan LIU
;
Jie MENG
;
Wen-Chao LI
;
Hui-Qi LI
;
Hua GUO
;
Mei-Lan NIU
;
Qiao-Hong QIN
Author Information
- Keywords: Siha cells; ARHGAP30; cervical cancer; gene knockdown; cell proliferation; cell apoptosis
- From: Chinese Pharmacological Bulletin 2024;40(5):847-853
- CountryChina
- Language:Chinese
- Abstract: Aim To investigate the changes in the proliferation and apoptosis of Siha cells after knocking down Rho GTPase-activating protein 30(ARHGAP30).Methods After designing specific shARHGAP30 primers and connecting them to the pLKO.1 vector,we transformed them into Escherichia coli competent cells,then co-transfecting them with lentiviral helper plasmids into HEK-293T cells.We collected and filtered cell supernatant to obtain the vi-rus to infect Siha cells.RT-qPCR and Western blot were used to detect knockdown efficiency,as well as changes in the expression of Bax and Bcl-2 after trans-fection.The CCK-8 method was employed to measure the proliferation level of cells after knockdown.Results After successful construction of a lentiviral plasmid with knockdown of the ARHGAP30 gene and establish-ment of stably transfected Siha cells,ARHGAP30 tran-scription and translation(P＜0.01)in Siha cells de-creased,Bax/Bcl-2 significantly decreased(P＜0.01),indicating decreased apoptosis and increased cell proliferation(P＜0.01).Conclusions This study suggests the involvement of ARHGAP30 in the proliferation and apoptosis of Siha cells,and regulating the ARHGAP30 gene may interfere with the occurrence and development of cervical cancer.
