miR-23a targets the XIAP-caspase-3 signaling pathway to contribute to the effects of resveratrol on ovarian function in mice
10.12007/j.issn.0258-4646.2024.11.013
- VernacularTitle:白藜芦醇通过miR-23a靶向XIAP-caspase-3信号通路影响小鼠卵巢功能
- Author:
Yinling XIU
1
;
Yuexin YU
;
Kaixuan SUN
;
Jing ZHOU
;
Panpan ZHAO
;
Jinlong XU
Author Information
1. 中国人民解放军北部战区总医院生殖医学科,沈阳 110003
- Keywords:
resveratrol;
premature ovarian failure;
miR-23a;
X-linked inhibitor of apoptosis protein;
caspase-3
- From:
Journal of China Medical University
2024;53(11):1031-1035
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of resveratrol(RES)on ovarian function in mice and elucidate the potential mechanism involving miR-23a.Methods Thirty mice were randomly divided into control,premature ovarian failure(POF)model,and treatment(RES)groups,with n=10 per group.The body weight and ovarian mass of the mice were measured,and the ovarian index was calculated.Hematoxylin and eosin staining was performed to observe pathological changes in mouse ovarian tissue.Real-time quantitative PCR and Western blotting were performed to measure the mRNA and protein levels of miR-23a,X-linked inhibitor of apoptosis protein(XIAP),and caspase-3 in the ovarian tissue.Results Compared with the control group,the POF group exhibited significant decreases in ovarian mass(P＜0.05),ovarian index(P＜0.05),number of primary follicles,and XIAP mRNA expression(P＜0.05),alongside significant increases in miR-23a and caspase-3 mRNA expression(P＜0.05).Compared with the POF group,the RES group exhibited significant increases in the ovarian mass(P＜0.05),ovarian index(P＜0.05),number of primary follicles,and XIAP mRNA expression(P＜0.05),as well as significant decreases in miR-23a and caspase-3 mRNA expression(P＜0.05).XIAP protein expression was significantly lower and caspase-3 protein expression was significantly higher in the POF group than in the control group(P＜0.05).Conversely,XIAP protein expression was significantly higher and caspase-3 protein expression was significantly lower in the RES group than in the POF group(P＜0.05).Conclusion RES exerts a protective effect on weakened ovarian function in mice,potentially mediated through its effect on miR-23a targeting the XIAP-caspase-3 signaling pathway.
