Mechanism underlying ITGB1-induced drug resistance in gastric cancer based on the circRNA regulatory network
10.12007/j.issn.0258-4646.2024.10.009
- VernacularTitle:基于circRNA调控网络探讨ITGB1诱导胃癌耐药的作用机制
- Author:
Yong CHEN
1
;
Donglei HE
;
Jianghao ZHOU
;
Yuexiang LIANG
;
Cheng YANG
Author Information
1. 海南医科大学第一附属医院胃肠肿瘤外科,海口 570102
- Keywords:
circRNA regulatory network;
gastric cancer;
oxaliplatin;
drug resistance
- From:
Journal of China Medical University
2024;53(10):923-928,938
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the mechanism underlying ITGB1-induced drug resistance in gastric cancer based on the circRNA-miRNA-ITGB1 regulatory network.Methods Tumor tissue samples were collected from 21 patients with gastric cancer.The ITGB1 gene expression levels were determined using real-time fluorescent quantitative polymerase chain reaction,and circRNA sequencing was performed to compare the differences in circRNAs between patients with low and high ITGB1 expression.BGC-823 cells were trans-fected with si-circ_0027189,si-miR-455,or si-NC and divided into the si-circ_0027189,si-miR-455,or si-NC groups,respectively.The circ_0027189,miR-455,and ITGB1 expression levels in each group and the sensitivity to oxaliplatin were measured.Results The circRNA regulatory network showed that circ_0027189 regulated ITGB1 expression through miR-455.Compared to the si-NC group,the si-circ_0027189 group exhibited decreased expression levels of circ_0027189 and ITGB1,increased expression levels of miR-455,and reduced sensitivity to oxaliplatin.In contrast,the si-miR-455 group showed decreased expression levels of miR-455,increased expression levels of ITGB1,and enhanced sensitivity to oxaliplatin compared to the si-NC group.Conclusion circ_0027189 can increase ITGB1 expression levels by targeting miR-455 expression,ultimately increasing drug resistance in gastric cancer cells.
