Long non-coding RNA OIP5-AS1 promotes epithelial-mesenchymal transition in colorectal cancer cells
10.12007/j.issn.0258-4646.2024.10.003
- VernacularTitle:长链非编码RNA OIP5-AS1促进结直肠癌细胞上皮-间质转化
- Author:
Jian SUN
1
;
Jiankang YU
;
Yanxi DUAN
;
Jianping ZHOU
Author Information
1. 中国医科大学附属第一医院胃肠外科/疝与腹壁外科,沈阳 110001
- Keywords:
colorectal cancer;
OIP5-AS1;
epithelial-mesenchymal transition;
metastasis
- From:
Journal of China Medical University
2024;53(10):877-881
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of the long non-coding RNA,Opa interacting protein 5-antisense RNA 1(OIP5-AS1),on colorectal cancer metastasis and elucidate the underlying mechanisms.Methods OIP5-AS1 expression levels were analyzed in various colorectal cancer cell lines(SW480,HCT116,HT-29,and Caco-2)via real-time polymerase chain reaction.Cell lines with high OIP5-AS1 expression levels were selected and randomly transfected with the control sequence(NC group),interference sequence 1(OIP5-AS1 siRNAl group),or interference sequence 2(OIP5-AS1 siRNA2 group).Transwell assay was used to assess cell migration.Additionally,the effects of OIP5-AS1 on the expression levels of epithelial-mesenchymal transition-related proteins,including E-cadherin,N-cadherin,and Vimentin,were examined via Western blotting.Immunofluorescence assay was used to evaluate the effects of OIP5-AS1 on the pro-tein expression levels and cellular localization of E-cadherin and N-cadherin.Results Expression levels of OIP5-AS1 were significantly higher in the SW480 and HCT116 highly invasive cell lines than in the HT-29 and Caco-2 low invasive cell lines(P＜0.05).OIP5-AS1 silencing decreased cell migration,increased E-cadherin levels,and decreased N-cadherin and Vimentin levels in SW480 and HCT116 cells.Conclusion Our findings suggest that OIP5-AS1 promotes metastasis by inducing epithelial-mesenchymal transition and migration of colorectal cancer cells.
