Mechanism of Ginkgo biloba extract in alleviating myocardial injury after myocardial infarction:a bioinformatics and neutrophil extracellular trap study
10.12007/j.issn.0258-4646.2024.10.002
- VernacularTitle:基于生物信息学和中性粒细胞胞外诱捕网效应探讨银杏叶提取物减轻心肌梗死后心肌损伤的机制
- Author:
Dongsheng WEI
1
,
2
;
Yuting LIU
;
Han LI
;
Jiajie QI
;
Xiaosheng LIU
;
Zhe ZHANG
Author Information
1. 辽宁中医药大学研究生院,沈阳 110847
2. 辽宁中医药大学中医脏象理论及应用教育部重点实验室,沈阳 110847
- Keywords:
myocardial infarction;
Ginkgo biloba extract;
neutrophil extracellular trap
- From:
Journal of China Medical University
2024;53(10):870-876
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the mechanism by which Ginkgo biloba extract reduces myocardial injury in rats with myocardial infarction(MI).Methods Bioinformatics was used to identify key targets of Ginkgo biloba extract in the treatment of MI in rats.An MI rat model was established via coronary artery ligation.Rats were randomly assigned to the model group,Betaloc ZOK group(2.5 mg/kg),and low-,medium-,and high-dose Ginkgo biloba extract groups(50,100,and 200 mg/kg,respectively).A sham-operation group was included for comparison.Following six weeks of gavage administration,myocardial tissues were examined using hematoxylin-eosin and Sirius red staining to assess pathological changes.Wheat germ agglutinin staining was used to observe cardiomyocyte hypertrophy.Western blotting was performed to evaluate changes in the expression of MCODE core targets,including HIF1α,MAPK14,MMP9,and CXCR4.Results Compared to the sham-operation group,the model group exhibited irregular arrangement of cardiomyocytes,significant inflam-matory infiltration,and a marked increase in type Ⅰ and Ⅲ collagen fibers in the myocardial tissue(P＜0.05).Additionally,significant upregulation in the expression of MCODE core target proteins(P＜0.05)was observed.In contrast,compared to the model group,low-,medium-,and high-dose Ginkgo biloba extract groups displayed more orderly arrangement of cardiomyocytes,reduced inflammatory infil-tration,significantly decreased levels of type Ⅰ and Ⅲ collagen fibers(P＜0.05),notable downregulation in the expression of p-MAPK14,MMP9,and CXCR4 proteins(P＜0.05),and significantly increased expression of HIF1α protein(P＜0.05).Conclusion Ginkgo biloba extract may exert protective effects on myocardial cells following MI by inhibiting the formation of neutrophil extracellular traps.
