Expression and significance of autophagy-related gene 5 in human malignant pleural mesothelioma
10.12007/j.issn.0258-4646.2024.08.001
- VernacularTitle:自噬相关基因5在人恶性胸膜间皮瘤中的表达及其意义
- Author:
Ruai LIU
1
,
2
,
3
;
Boyong WANG
;
Jinsong LI
;
Yuanqian PU
;
Min YU
;
Wei XIONG
Author Information
1. 大理大学基础医学院生物化学与分子生物学教研室,云南 大理 671000
2. 云南省高校临床生物化学检验重点实验室,云南 大理 671000
3. 云南省昆虫生物医药研发重点实验室,云南 大理 671000
- Keywords:
malignant pleural mesothelioma;
autophagy-related gene 5;
autophagy;
prognosis;
immune infiltration
- From:
Journal of China Medical University
2024;53(8):673-679
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the expression of autophagy-related gene 5(ATG5)in human malignant pleural mesothelioma(MPM)cells and tissues and analyze the correlation between ATG5expression and patient clinicopathological parameters and prognosis.Methods Real-time quantitative PCR and Western blotting were used to detect differences in ATG5 expression between normal human pleural mesothelial cells and MPM cells and between non-MPM pleural mesothelial tissues and MPM tissues.The Cancer Genome Atlas was used to analyze correlations between ATG5 expression and clinicopathological characteristics and prognosis of patients with MPM.A Cox proportional hazard model was constructed to analyze factors affecting the prognosis of patients with MPM.The Gene Expression Profiling Interactive Analysis database was used to evaluate the correlation between ATG5and MPM tumor markers and novel serum markers.Tumor Immune Estimation Resource was used to analyze correlations between ATG5 and MPM immune cell infiltration and key immune regulatory genes.Results ATG5 was highly expressed in MPM cells and tissues and positively correlated with tumor stage(P<0.05).High ATG5 expression indicated poor prognosis(P<0.05).ATG5expression was significantly associated with various MPM tumor markers(MTAP,SETD2,NF2,and FIB3)and novel serum markers(HMGB1,SMPR,THBS2,and KRAS)(P<0.05).ATG5was associated with immune cell infiltration in MPM(B cells,CD4+T cells,and macrophages)and expression of immune-related genes(CD28,CUL48B,CD166,and MMP14)(P<0.05).Conclusion ATG5 is upregulated in MPM and is associated with poor prognosis and immune cell infiltration.ATG5 could be a key biomarker for early screening,diagnosis,and prognostic assessment of MPM.
