Effect of long non-coding RNA FGD5-AS1 on high glucose-induced myocardial cell injury through regulation of the miR-195-5p/PIM1 axis
10.12007/j.issn.0258-4646.2024.06.002
- VernacularTitle:长链非编码RNA FGD5-AS1调节miR-195-5p/PIM1轴对高糖诱导的心肌细胞损伤的影响
- Author:
Bing TAN
1
;
Lingyan FANG
;
Minghua CHEN
;
Qiaoli ZENG
;
Runmin GUO
Author Information
1. 广东医科大学 附属第二医院内分泌科,广东 湛江 524000
- Keywords:
lncRNA FGD5-AS1;
miR-195-5p/PIM1;
high glucose;
myocardial cell;
injury
- From:
Journal of China Medical University
2024;53(6):487-494
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of the long non-coding RNA FGD5 antisense RNA1(lncRNA FGD5-AS1)on myocar-dial cell injury induced by high glucose levels through regulation of the miR-195-5p/Pim-1 proto-oncogene(PIM1)axis.Methods The rat myocardial cell line H9c2 and human myocardial cells were randomly allocated to the control,model,lncRNA FGD5-AS1 overex-pression,miR-195-5p inhibitor,negative control,and lncRNA FGD5-AS1 overexpression+miR-195-5p mimic groups to determine the expression of lncRNA FGD5-AS1,miR-195-5p,and PIM1.In addition,cell proliferation,apoptosis,and pro-inflammatory cytokine levels of the cells in each group were analyzed.Results Compared with the control group,the protein and mRNA expressions of lncRNA FGD5-AS1 and PIM1 and the survival and proliferation rates in H9c2 and human myocardial cells in the model group decreased(P<0.05),and the expression of miR-195-5p,apoptosis rate,and production levels of tumor necrosis factorα(TNF-α)and interleukin-6(IL-6)increased(P<0.05).Overexpression of the lncRNA FGD5-AS1 reversed these pathological changes in model group cells,whereas upregulation of miR-195-5p could weaken the effects of overexpression of lncRNA FGD5-AS1.Conclusion Overexpression of lncRNA FGD5-AS1 enhances the expression of PIM1 by downregulating miR-195-5p,thereby inhibiting high glucose-induced myocyte inflamma-tion,promoting survival and growth,and alleviating apoptotic injury.
