Expression and clinical significance of basement membrane genes in endometrial carcinoma
10.12007/j.issn.0258-4646.2024.05.011
- VernacularTitle:基底膜基因在子宫内膜癌中的表达及其临床意义
- Author:
Congyu YANG
1
;
Qingchun DENG
;
Jiaming LIU
Author Information
1. 蚌埠医科大学临床医学院,安徽 蚌埠 233030
- Keywords:
basement membrane;
endometrial carcinoma;
extracellular matrix
- From:
Journal of China Medical University
2024;53(5):451-457
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the expression levels and clinical significance of basement membrane genes in endometrial carcinoma and the prognostic value of a prediction model.Methods The mRNA expression matrix and clinical data for endometrial carcinoma were obtained from The Cancer Genome Atlas(TCGA)database,and basement membrane genes were obtained from a previous study.Prognostic genes were screened using univariate and multivariate Cox regression analyses based on differentially expressed genes(DEGs).A prognostic model was constructed,and the relationship of these genes with clinical factors was analyzed.The biological functions and pathways of the DEGs were assessed using functional enrichment analyses.The mRNA expression of prognostic genes was verified using real-time polymerase chain reaction(PCR).Results The prognostic model composed of ACHE,COL12A1,and CD44was significantly associated with clinical factors.Real-time PCR results showed that the expression levels of ACHEand COL12A1mRNA differed signifi-cantly between normal endometrial cells and endometrial carcinoma cells(P<0.05).Conclusion The constructed model based on three basement membrane genes can accurately predict the prognosis of patients with endometrial carcinoma and provide potential targets for clinical treatment.
