Changes in the protein expression profiles of glomeruli during the treatment of lupus nephritis with hydroxychloroquine sulfate
10.12007/j.issn.0258-4646.2024.05.003
- VernacularTitle:硫酸羟氯喹治疗狼疮性肾炎过程中肾小球内蛋白质表达谱的变化
- Author:
Yanqiu LI
1
;
Haichao WANG
;
Xiaojie ZHENG
;
Xiaodan LIU
;
Xue LIU
;
Shuyan TIAN
;
Li YAO
Author Information
1. 中国医科大学附属第一医院肾脏内科,沈阳 110001
- Keywords:
glomerulus;
lupus nephritis;
proteomics;
two-dimensional electrophoresis
- From:
Journal of China Medical University
2024;53(5):401-404,413
- CountryChina
- Language:Chinese
-
Abstract:
Objective To screen the differentially expressed proteins in glomeruli during the treatment of lupus nephritis(LN)with hydroxychloroquine sulfate(HCQ).Methods Sixty female NZB/WF1 mice were used.At the age of 28 weeks,40 mice with proteinuria of 3+to 4+were divided into HCQ and control groups.After feeding for 36 weeks,the glomerular proteins were extracted by magnetic beads and analyzed by two-dimensional fluorescence difference gel electrophoresis(2D-DIGE)and matrix-assisted laser desorption/ioniza-tion time-of-flight mass spectrometry(MALDI-TOF-MS).The expression of the candidate proteins in the glomeruli of the LN mice was exa-mined by immunohistochemistry.Results A total of 31 differentially expressed proteins were identified between the two groups,including 24 upregulated and seven downregulated proteins.Conclusion The expression of proteins like RI and ENOA in the glomeruli of LN mice was significantly different during HCQ treatment.These proteins may be involved in the pathogenesis of LN and are therefore potential targets of HCQ in the treatment of LN.
