Effect of Staphylococcal Toxic Shock Syndrome Toxin-1 on Secretion of Interleukins and Interferon-gamma by Peripheral Blood Mononuclear Cells from Atopic Donors.
- Author:
Jong Kyu YANG
;
Sang Chin LEE
;
Si Yong KIM
;
Chung Won KIM
- Publication Type:In Vitro ; Original Article
- Keywords:
Spinal Stenosis with Spondylolisthesis;
Reduction;
Transpedicular Screw Systems;
Posterolateral Fusion
- MeSH:
Cytokines;
Dermatitis, Atopic;
Enterotoxins;
Healthy Volunteers;
Humans;
Interferon-gamma*;
Interleukin-10;
Interleukin-12;
Interleukin-4;
Interleukins*;
Shock, Septic*;
Skin;
Skin Diseases;
Staphylococcus aureus;
Tissue Donors*
- From:Korean Journal of Dermatology
1998;36(3):385-390
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: The majority of Staphylococcus aureus strains isolated from atopic eczema skin produce toxins like enterotoxin B or toxic shock syndrome toxin-1(TSST-1) as a superantigen and these toxins may contribute to the exacerbation of this skin disease. OBJECTIVE: We designed this study in order to investigate the effect of toxic shock syndrome toxin-1 on IL-4, interleukin-10(IL-10), interleukin-12(IL-12) and IFN- r production by peripheral blood mononuclear cells from normal and atopic patients. METHODS: Peripheral mononuclear cells obtained from ten patients with severe atopic dermatitis and ten healthy volunteers were stimulated with TSST-1 and cultured for 4 days. The amount of IL-4, IL-10, IL-12 and IFN- r in the culture supernatants were determined by a solid-phase enzyme- linked immunosorbant assay. RESULTS: l. After stimulation with TSST-1, IL-4, IL-10, IL-12 and INF- r, production of peripheral mononuclear cells from both atopic patients and healthy volunteers were increased. 2. After stimulation with TSST-1, the amount of IFN- r in the culture supernatants was significantly greater in the healthy controls than in the atopic patients. 3. After stimulation with TSST-1, the amount of IL-10 in the culture supernatants was significantly greater in the healthy controls than in the atopic patients. CONCLUSION: This data indicates that TSST-1 induces Thl-type and Th2-type cytokines associated with parallel Thl and Th2 stimulation in atopic patients and healthy donors. In addition, peripheral mononuclear cells from atopic patients have a diminished capacity to synthesize IFN- r and IL-10 in vitro in response to stimulation with TSST-1. Our results suggest that TSST-1 is not considered as a really important aggravating factor in atopic dermatitis.
