RP-HPLC Determination of Simvastatin and Its Related Substance Lovastatin
10.3969/j.issn.1007-7693.2007.05.013
- VernacularTitle:反相高效液相色谱法测定辛伐他汀及其有关物质洛伐他汀的含量
- Author:
Xue-Jun WANG
1
;
Zhen-Liang XU
;
Jian-Li FENG
;
Nai-Ci BING
;
Zuo-Guo YANG
;
Jin-Ping WEI
Author Information
1. 华东理工大学
- Keywords:
HPLC;
simvastatin;
lovastatin;
determination
- From:
Chinese Journal of Modern Applied Pharmacy
2007;24(5):383-385
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To establish a RP-HPLC method for determination of simvastatin and its related substance lovastatin. METHODS The chromatographic conditions were: a Waters Symmetry C18 column (250 mm×4.6 mm, 5 μm), a mixture of acetonitrile-sodium dihydrogen phosphate (pH 5.4) (65∶35) as mobile phase, flow rate 1.0 mL·min-1, and detected at 238 nm. RESULTS The linear ranges of lovastatin and simvastatin were 0.3～3.0 μg·mL-1,0.03～0.30 mg·mL-1, respectively. The average recovery were 100.2% (RSD=1.5%) and 99.4% (RSD=1.7%), respectively. CONCLUSION The method is simple, quick, sensitive, accurate, and reproducible. It can be used to the quality control of synthetic simvastatin products.
