Measurement of cyclosporine A in aqueous humor of rabbit eyes by HPLC
10.3969/j.issn.1007-7693.2005.04.012
- VernacularTitle:反相高效液相色谱法测定兔眼房水环孢素A
- Author:
Ai-Ming LIU
1
;
Ju-Lin YANG
;
Zhi-Hua LÜ
Author Information
1. Shandong Eye Hospital and Institute
- Keywords:
cyclosporine A;
HPLC;
aqueous humor of rabbit eyes
- From:
Chinese Journal of Modern Applied Pharmacy
2005;22(4):301-303
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To develop a simple and reproducible high-performance liquid chromatography (HPLC) method for determination of cyclosporine A (CsA, also known as cyclosporin A) in aqueous humor of rabbit eyes using CsD as internal standard. METHODS The pretreatment involved one step of liquid- liquid extraction with ether from aqueous humor samples. Chromatography was carried out using an ODS column which was controlled at 75℃ under isocratic elution with the mobile phase of acetonitrile-deionized water (89:11, v/v)at the flow rate of 0.1mL·min-1 and the detector set at 200 nm. RESULTS The mean absolute recovery of CsA from aqueous humor was 86% and the mean relative recovery was 100.7%.The linearity was assessed in the range of 25～500 ng·mL-1, with a correlation coefficient of 0.9986. The limit of quantification and detection of the present method were 25 and 10 ng·mL-1, respectively. CONCLUSION This method has been used to analyze large numbers of aqueous humor samples of rabbit eyes for ophthalmic research and proved to be rapid with good accuracy and precision.
