Determination of main constituents in ofloxacin and ephedrine nasal dro ps by RP-HPLC
10.3969/j.issn.1007-7693.2001.02.017
- VernacularTitle:反相高效液相色谱法测定泰麻滴鼻剂中主药的含量
- Author:
Zhiqiang WANG
1
;
Xuesun HUANG
;
Xiuhua ZHANG
Author Information
1. The Frist Affilated Hospital Wenzhou Medic al College
- From:
Chinese Journal of Modern Applied Pharmacy
2001;18(2):98-99
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To deve lop an assay for ofloxacin and ephedrine in ofloxacin and ephedrine nasal drops. METHODS:A RP-HPLC methods was practised on a hypersil ODS column with a mobile phase of methanol-10g/L ammonium acetate in water(1:1),which pH w as adjuested to 6.0 with phosphoric acid.Chloramphenicol was used as the interna l standand,UV detection was performed at 256nm.REULTS:The standand c urves for ofloxacin and ephedrine were linear in the range of 20～100μg/ml(r=0.9999,n=5),100～1000μg/ml(r=0.9999,n=5),respectively. Th e average recoveries of ofloxacin and ephedrine were 99.5%,101.5% respectively. CONCLUSION:The method was convenient,rapid and accurate.
