Effect of anti-PD-1 treatment on the immune microenviron-ment in patients with colorectal cancer
10.3969/j.issn.1009-9905.2024.10.003
- VernacularTitle:PD-1单抗治疗对微卫星不稳定型直肠癌免疫微环境的影响
- Author:
Hao WANG
1
;
Ming ZHOU
;
Hao-Yu SHI
;
Si-Kun LIU
;
Biao SHENG
;
Xiao-Xu GE
;
Jian WANG
Author Information
1. 浙江大学医学院附属第二医院 大肠外科(浙江 杭州 310009)
- Keywords:
Colorectal neoplasms;
Single-cell mass cytometry;
PD-1 blockers;
Tumor micro-environment
- From:
Chinese Journal of Current Advances in General Surgery
2024;27(10):766-772
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effects of PD-1 monoclonal antibody therapy on the peripheral and local immune microenvironment of patients with microsatellite instability-high(MSI-H)rectal cancer.Methods:Samples of peripheral blood and tumor biopsy were collected from a patient with MSI-H rectal cancer before and after PD-1 monoclonal antibody treatment.The samples were dissociated into single-cell suspensions using a combination of enzymatic and mechanical methods.Immune-related marker expression on peripheral and tumor-infiltrating im-mune cells was analyzed using single-cell mass cytometry(CyTOF).Results:According to the results of CyTOF analysis,CD45+immune cells in the peripheral blood and tumor tissues were categorized into 39 and 34 cell subsets,respectively,before and after PD-1 monoclonal antibody treatment(the correlation is unclear and ambiguous).After PD-1 monoclonal antibody treatment,differences were observed in the relative abundance of immune cell subsets:B cells significantly decreased in the peripheral blood,while B cells and γδT cells significantly increased in the tumor tissue;neutrophils significantly decreased,and the proportion of CD4+TEM cells in T cell subsets significantly increased,whereas CD4+Treg cells significantly decreased.Additionally,there were differences in the expression of immune-related markers in multiple immune cell subsets in both peripheral blood and tumor tissues,with CCR6 showing a significant increase in expression across all subsets,while ICOS and PD-1 expressions in T cell subsets were significantly reduced(the specific tissues for these cells or factors are unclear).Conclusion:After PD-1 monoclonal antibody treatment in MSI-H rectal cancer,changes occurred in the composition of immune cells and the expression of immune-related markers in both peripheral blood and tumor tissues.This study reveals the dynamic adjustment of the immune microenvironment and provides important evidence for understanding the therapeutic mechanism of PD-1 monoclonal antibodies.
