Expression of CRKL in intrahepatic cholangiocarcinoma and its effect on the proliferation and invasion of cholangiocarci-noma cells
10.3969/j.issn.1009-9905.2024.09.003
- VernacularTitle:CRKL在肝内胆管癌中的表达及对癌细胞增殖和侵袭的影响
- Author:
Wen-Lei KUO
1
;
Xin-Yue BAO
;
Jing-Bo CHANG
;
Qi SUN
;
Li-Min WEI
Author Information
1. 西安交通大学第一附属医院 普通外科(陕西 西安 710061)
- Keywords:
Intrahepatic cholangiocarcinoma;
CRKL;
Cell proliferation;
Cellinvasion;
AKT signal-ing pathway;
ERK signaling pathway
- From:
Chinese Journal of Current Advances in General Surgery
2024;27(9):684-688
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the expression of kinase-like gene CT10 regulatory fac-tor(CRKL)in intrahepatic cholangiocarcinoma(ICC)and its effect on the proliferation and invasion of intrahepatic cholangiocarcinoma cells.Methods:qRT-PCR detected CRKL transcription in ICC pa-tient tissues and adjacent tissues.Immunohistochemistry assessed CRKL expression in ICC tissues and adjacent tissues,and analyzed its relationship with clinicopathological features.For the inhibition of CRKL expression in QBC939 cells by siRNA technology,the effect of CRKL silencing on AKT and ERK signaling pathway was measured by Western blot.CRKL's influence on QBC939 cell prolifera-tion and invasion was analyzed by MTT,clonogenesis,and Transwell assays.Results:The expres-sion of CRKL in ICC tissues was up-regulated,and there were statistically significant differences in CRKL expression in ICC with different clinicopathological features such as tumor size,lymph node metastasis and TNM stage.Cellular experiments showed a significant decrease in the phosphoryla-tion of AKT and ERK upon inhibition of CRKL,and the proliferation and invasion ability of ICC cells was significantly diminished(P＜0.05).Conclusion:CRKL promotes ICC cell proliferation and invasion through AKT and ERK pathways,offering new molecular targets and directions for targeted therapy.
