Identification of genes related to prognosis of retroperi-toneal liposarcoma based on transcriptome sequencing
10.3969/j.issn.1009-9905.2024.04.008
- VernacularTitle:转录组测序技术识别腹膜后脂肪肉瘤预后相关基因
- Author:
Chang-Chun YE
1
;
Miao-Miao CHI
;
Yan-Sheng HU
;
Ge LI
;
Xue-Jun SUN
;
Qi SUN
Author Information
1. 西安交通大学第一附属医院 普通外科(陕西 西安 710061)
- Keywords:
Retroperitoneal neoplasms;
Transcriptome sequencing;
Differentially expressed genes;
Prognosis
- From:
Chinese Journal of Current Advances in General Surgery
2024;27(4):292-297
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To analyze and compare the differences of expression profiles between RPL and normal adipose tissue by transcriptome sequencing(RNA-Seq),then identify the key genes related to prognosis and explore their potential mechanisms.Methods:Tumor tissues and normal adipose tissues of patients with RPL were collected for RNA-Seq,and then the differentially ex-pressed genes were analyzed by GO and KEGG enrichment analysis.Based on TCGA,the high-risk genes related to prognosis were screened and verified by Kaplan-Meier curve and receiver operat-ing characteristic(ROC)curve.Results:Compared with normal adipose tissue,279 differentially expressed genes were simultaneously up-regulated in RPL tissues,which were mainly enriched in immune response and PPAR signaling pathway.Combined with TCGA,7 stable prognostic high risk genes were identified and the overall survival rate of the high risk group was significantly lower than that of the low risk group(P＜0.05).Conclusion:KCNQ5,RBPJ and some other genes may be re-lated to the poor prognosis of RPL patients.The analysis of the mechanism of these genes in RPL is expected to provide new evidence for the formulation of diagnosis and treatment strategies for RPL patients.
