Analysis of key genes in the development from colon adeno-ma to carcinoma through high-throughput RNA sequencing
10.3969/j.issn.1009-9905.2024.04.007
- VernacularTitle:高通量RNA测序技术分析结肠腺瘤转变为结肠癌的关键基因
- Author:
Jie BIAN
1
;
Tao WANG
;
Chang-Chun YE
;
Gen-Wang GAO
;
Chun-Hong MA
;
Xue-Jun SUN
;
Qi SUN
Author Information
1. 西安交通大学第一附属医院 普通外科(陕西 西安 710061)
- Keywords:
Colon carcinoma;
Colon adenoma;
RNA sequencing
- From:
Chinese Journal of Current Advances in General Surgery
2024;27(4):286-291
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To analyze and compare the difference of gene expression profiles in normal colon tissues,colon adenoma and carcinoma tissues by RNA sequencing technology,and re-veal the key genes and potential mechanisms in the development from colon adenoma to carcinoma.Methods:RNA sequencing analysis was carried out on normal colon tissues,colon adenomas and carcinoma tissues of the same patient,and differential genes that were significantly expressed in colon cancer and not significantly expressed in adenoma tissues were obtained,and the GO and KEGG function enrichment analysis was performed.Results:There are 4307 differential genes that are significantly expressed in colon cancer and not significantly expressed in adenoma.The GO and KEGG function enrichment analysis of these genes found that they were mainly enriched in bi-ological processes such as biological process regulation,cell process regulation,protein binding and cancer pathway,PI3K Akt signal pathway MAPK signal pathway.Conclusion:There are many genes involved in the development process from colon adenoma to carcinoma.These genes have the potential to become therapeutic targets for colorectal cancer,providing a new direction for fol-low-up research on colorectal cancer.
