Research on the effect of fibroblast exosomes from diabetic rats on wound healing
10.3969/j.issn.1006-6187.2024.11.008
- VernacularTitle:糖尿病大鼠来源成纤维细胞外泌体对创面愈合影响的研究
- Author:
Hongrui CHEN
1
;
Xiaofan YANG
;
Yu KANG
;
Jiahe GUO
;
Zhenbing CHEN
Author Information
1. 430022 武汉,华中科技大学同济医学院附属协和医院病理科
- Keywords:
Diabetic;
Exosomes;
Wound healing;
Fibroblasts
- From:
Chinese Journal of Diabetes
2024;32(11):849-855
- CountryChina
- Language:Chinese
-
Abstract:
Objective To expore the effect of fibroblast exosomes from diabetic rats on wound healing.Methods Male SD rats were randomly divided into diabetes mellitus group(DM group,n=12)and normal control group(NC group,n=15).Fibroblast exosomes were extracted and identified.After digestion of human epidermal keratinocyte line(HEK-a)and human epidermal microvascular endothelial cell line(HMEC-1),the cells were divided into diabetes exosomes group(DM-EOXa group),normal exosomes group(N-EXOa group)and normal control group(NCa group).CCK8 was used todetect the proliferation of fibroblast.fifteen male SD rats were randomly divided into diabetes exosomes group(DM-EXOb group),normal exosomes group(N-EXOb group)and normal control group(NCb group),with 5 rats in each group.Full-thickness wounds were created on rats back,and fibroblast exosomes were used to intervene wound healing.The wound healing was compared on 3th,7th,10th,and 14th day in each group.Results The proliferation of HEK-a and HMEC-1 was higher in N-EXOa and DM-EXOa groups than in NCa group(P<0.05).On the 7th,10th and 14th day,the average wound rate was lower in N-EXOb group than in NCb and DM-EXOb groups(P<0.05 or P<0.01).On the 10th day,the average wound rate was lower in NCb group than in DM-EXOb group(P<0.05).Conclusions Normal fibroblast exosomes can promote wound healing,while diabetic fibroblast exosomes from diabetic rats have no effect on wound healing,and even inhibit wound healing.
