Effect of silencing lncRNA TUG1 on pyroptosis induced by high glucose in microglia
10.3969/j.issn.1006-6187.2024.10.009
- VernacularTitle:沉默长链非编码RNA牛磺酸上调因子1对高糖诱导的小胶质细胞焦亡作用机制的研究
- Author:
Youzhuo PAN
1
;
Wenhui GUO
;
Haoyue LEI
;
Xun LU
;
Qi ZHANG
Author Information
1. 730000 兰州,甘肃中医药大学第一临床医学院
- Keywords:
BV2;
lncRNA TUG1;
Pyroptosis;
GSDMD
- From:
Chinese Journal of Diabetes
2024;32(10):764-769
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the mechanism of action of the long non-coding RNA(lncRNA)taurine up-regulating factor 1(TUG1)with high glucose(HG)-induced cellular pyroptosisin microglial cell.Methods Mouse BV2 cells were cultured and divided into normal control(NC),HG,lentiviral empty vector(sh-Con),TUG1 lentiviral gene silencing vector(sh-TUG1),HG+sh-Con and HG+sh-TUG1 group.RT-PCR was used to detect the expression and transfection efficiency of TUG1 mRNA.Nucleotide-binding oligomerized structural domain-like receptor protein 3(NLRP3),cysteoaspartate protease-1(Caspase-1),and ghrelin D(GSDMD),IL-18,IL-1β mRNA and protein expression were detected by RT-PCR and Western blot.Results TUG1 mRNA expression was higher in HG group than in NC group(P＜0.05).After transfection,a lot of green fluorescence appeared in sh-TUG1 and sh-Con group,while no green fluorescence was observed in NC group.The expression of TUG1 mRNA was lower in sh-TUG1 group than in NC group(P＜0.05).Accordingly,the recombinant lentivirus successfully infected BV2 cell.The expressions of the mRNA and protein of NLRP3,Caspase-1,GSDMD,IL-18 and L-1β were higher in HG,HG+sh-Con groups than in NC group(P＜0.05).The expressions of the mRNA and protein of NLRP3,Caspase-1,GSDMD and IL-18 and L-1β were lower in HG+shTUG1 group than in HG,HG+sh-Con groups(P＜0.05).Conclusions TUG1 is involved in high glucose induced pyroptosis in microglia and leads to inflammatory response.Silencing TUG1 can inhibit the pathological reaction.
