Effect of resistant starch on intestinal flora and metabolism in obese rats with type 2 diabetes mellitus
10.3969/j.issn.1006-6187.2024.09.012
- VernacularTitle:抗性淀粉对2型糖尿病肥胖大鼠肠道菌群及代谢影响的研究
- Author:
Xuehui WU
1
;
Jun XUE
;
Jiali HE
Author Information
1. 014010 内蒙古科技大学包头医学院
- Keywords:
Diabetes mellitus,type 2;
Glycolipid metabolism;
Resistant starch;
Intestinal flora
- From:
Chinese Journal of Diabetes
2024;32(9):695-702
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of resistant starch(RS)on the intestinal microbiota and metabolism in obese rats with type 2 diabetes mellitus(T2DM).Methods The Wistar rats were divided into normal control(NC)group,model group(Mod),acarbose intervention group(Aca)and RS intervention group(RS),with 8 rats in each group.TC,TG,HDL-C,and LDL-C were detected after 8 weeks of continuous administration.Lee's index,insulin sensitivity index(HOMA-IS),insulin resistance index(HOMA-IR),pancreatic β cell function index(HOMA-β),inflammatory factors,and area under the curve of glucose tolerance(AUC)were calculated.Serum TNF-α,IL-6 and lipopolysaccharide(LPS)were detected by ELISA.The steatosis of liver tissue was observed by HE staining,and the feces were collected for 16S rDNA sequencing in each group.Species difference analysis,Beta diversity analysis and other methods were used to analyze the structure and quantity of intestinal microbiota.Results The Lee's index was higher in the Mod,Aca and RS groups than in the NC group(P<0.05).Compared with the NC group,TC,TG,LDL-C,HDL-C,FIns,HOMA-IR,TNF-α,IL-6 and LPS were increased(P<0.05),HOMA-β and HOMA-IS decreased in the Mod,Aca,and RS groups(P<0.05).Compared with the Mod group,TC,TG,LDL-C,HDL-C,FIns,HOMA-IR,TNF-α,IL-6 and LPS decreased(P<0.05),and HOMA-IS and HOMA-β increased in the Aca and RS groups(P<0.05).Compared with the Aca group,IL-6 was increased(P<0.05),and LDL-C and HDL-C decreased in the RS group(P<0.05).The results of 16S rDNA sequencing showed that the total number of operational taxonomic units were increased and the diversity and uniformity of the intestinal microbiota were also increased in the Aca and RS groups.Conclusions RS can regulate glucose and lipid metabolism,improve IR,increase intestinal microbial diversity,regulate intestinal microbiota structure,and maintain intestinal health in obese rats with T2DM.
