Liraglutide improves the spatial learning and memory function in rats with type 2 diabetes mellitus
10.3969/j.issn.1006-6187.2024.08.011
- VernacularTitle:利拉鲁肽改善2型糖尿病大鼠空间学习和记忆能力机制的研究
- Author:
Zhanying YE
1
;
Dai HUANG
;
Linquan YANG
;
Zhe ZHANG
;
Hanying XING
Author Information
1. 050051 石家庄,河北省人民医院代谢病重点实验室
- Keywords:
Diabetes mellitus,type 2;
Liraglutide;
Learning;
Memory;
Heme oxygenase 1
- From:
Chinese Journal of Diabetes
2024;32(8):617-622
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect and mechanism of Liraglutide on the spatial learning and memory function in type 2 diabetes mellitus(T2DM)rats.Methods SD rats were randomly divided into control group(NC),T2DM group(T2DM)and Liraglutide group(Lir).The ability of learning and memory was evaluated by Morris water maze method.Apoptosis of hippocampal neuron was tested by TUNEL staining.The mRNA expression levels of inflammatory cytokines TNF-α,IL-1β and IL-6 were measured by real time-PCR.The protein expressions of heme oxygenase-1(HO-1),NADPH oxidase 1(NOX1),protein B-cell lymphoma-2(Bcl-2)and Bcl2-associated X(Bax)in hippocampus tissues were determined by Western blot.Results Compared with T2DM group,the percent time in target quadrant was increased in Lir group(P<0.05),while the escape latency of day 3 to day 5 was significantly decreased in Lir group(P<0.05).The apoptosis of hippocampal neurons was reduced in Lir group,and the morphological changes,including hyperchromatic cytoplasm and karyopyknosis were alleviated.Compared with T2DM group,MDA content,mRNA level of TNF-α,IL-1β and IL-6,and protein expression of NOX1 and Bax were decreased(P<0.05),while T-AOC,SOD,HO-1 and Bcl-2 protein expression were increased in Lir group(P<0.05).Conclusions Liraglutide ameliorates diabetes-related learning and memory impairment by regulating antioxidant,anti-inflammatory and anti-apoptotic effects in hippocampus.
