Effect of adipocyte-specific Smc5 knockout on glucose and lipid metabolism
10.3969/j.issn.1006-6187.2024.06.009
- VernacularTitle:脂肪组织特异性染色体结构维持蛋白5基因敲除对小鼠糖脂代谢影响的研究
- Author:
Yuanping SHI
1
;
Ling YE
;
Meiling JIN
;
Wenjiao ZHU
Author Information
1. 100044 北京大学人民医院内分泌科
- Keywords:
CRISPR/Cas9;
Smc5;
Mice;
Adipose tissue;
Blood glucose
- From:
Chinese Journal of Diabetes
2024;32(6):443-449
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effectsof adipocyte-specific Structural maintenance of chromosomes 5(Smc5)knockouton glucose and lipid metabolism in mice.Methods Adipocyte-specific Smc5 knockout mice(AKO mice)were constructed based onclustered regularly interspaced short palindromic repeats/CRISPR-associated nuclease 9(CRISPR/Cas9)systems,and Smc5flox/flox mice(Flox mice)were used as controls.The Smc5 knockout efficiency of adipose tissue in mice was verified by qRT-PCR and Western blot.The body fat content was detected by dual energy X-ray absorptiometry(DEXA).The morphology of adipose tissue was observed by hematoxylin-eosin staining and the area distribution of adipocytes was calculated.TG,HDL-C,LDL-C,free fatty acid(FFA),intraperitoneal glucose tolerance test(IPGTT)and intraperitoneal insulin tolerance test(IPITT)were compared.Results The AKO mouse model of fat specific knockout of Smc5 gene was successfully constructed.Smc5 mRNA in groin fat(iWAT),epididymal fat(eWAT)and brown adipocyte(BAT)of AKO mice,body weight after 15 weeks,organ weight of iWAT at 31 weeks,organ weight of eWAT,organ weight of BAT,body weight,fat mass,fat mass/weight,LDL-C,and percentage of 15-minute blood glucose in IPITT were lower than those of Flox mice(P<0.05 or P<0.01).Conclusions Adipocyte-specific Smc5 gene knockout improves glucose and lipid metabolism by affecting adipose tissue production.
