To investigate the expression of lncRNA H19 in peripheral blood mononuclear cells type 2 diabetes mellitus complicated with osteoporosis
10.3969/j.issn.1006-6187.2024.05.010
- VernacularTitle:2型糖尿病合并骨质疏松症患者外周血单个核细胞长链非编码RNA H19表达的研究
- Author:
Teng YANG
1
;
Na WANG
;
Xiuli FENG
;
Tongyu ZHANG
;
Zhichen ZHENG
;
Guofeng WANG
Author Information
1. 222000 连云港,锦州医科大学连云港市第一人民医院研究生培养基地内分泌科
- Keywords:
Diabetes mellitus,type 2;
Osteoporosis;
Peripheral blood mononuclear cells;
Long non-coding RNA
- From:
Chinese Journal of Diabetes
2024;32(5):364-368
- CountryChina
- Language:Chinese
-
Abstract:
Objective Exploring the clinical application value of long chain non-coding RNA(lncRNA)H19 in peripheral blood mononuclear cells(PBMCs)in type 2 diabetes mellitus(T2DM)patients with osteoporosis(OP).Methods A total of 176 patients with T2DM admitted to the Department of Endocrinology of the First People's Hospital of Lianyungang City from December 2022 to June 2023 were selected.They were divided into OP group(n=100)and simple T2DM group(n=76)according to the results of bone mineral density(BMD)determination by dual-energy X-ray.General data and biochemical indicators were compared between the two groups.The correlation between lncRNA H19 and other clinical indicators was analyzed by Spearman correlation analysis and the influencing factors for T2DM with OP were analyzed by logistic regression.Receiver operating characteristic(ROC)curve was used to evaluate the diagnostic value of lncRNA H19 in T2DM with OP.Results Compared with T2DM group,the proportion of females,age,HbA1c,TC and TGF-β1 were higher(P<0.05),while BMI,SUA,BMD and lncRNA H19 were lower in OP group(P<0.05).Spearman correlation analysis showed that lncRNA H19 expression level was positively correlated with BMI,SUA and BMD(P<0.05),and negatively correlated with age,HbA1c and TGF-β1(P<0.05).Logistic regression analysis showed that age,BMI,TC,TGF-β1 and lncRNA H19 were the influencing factors for T2DM combined with OP(P<0.05).ROC curve analysis showed that the AUC of lncRNA H19 in the diagnosis of T2DM with OP was 0.839,the sensitivity was 76.3%,and the specificity was 86.0%.Multiple linear regression analysis showed that age,TGF-β1 and OP were the influencing factors for lncRNA H19(P<0.05).Conclusion LncRNA H19 expression decreased in PBMCs in patients with T2DM with OP,which may participate in the occurrence and development of T2DM with OP through glucose metabolism and lncRNA H19/TGF-β1 pathway.
