RHD Genotyping Characteristics of RhD-Negative Blood Donors in Wuhu Area
10.19746/j.cnki.issn1009-2137.2024.05.035
- VernacularTitle:芜湖地区初筛RhD阴性献血人群RHD基因分型特征
- Author:
Meng-Nan LI
1
;
Zhen-Jun DU
;
Jing-Wen LIU
;
Rui ZHANG
;
Yuan WANG
;
Dian-Ming CAO
;
Ji-Chun TAO
;
Lu-Chen ZOU
;
Hui HUANG
;
En-Tao SUN
Author Information
1. 皖南医学院公共卫生学院,安徽芜湖 241002
- Keywords:
Rh blood type;
RHD gene;
D variant;
Sanger sequencing
- From:
Journal of Experimental Hematology
2024;32(5):1531-1538
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the molecular mechanism and distribution characteristics of RhD negative phenotypes in Han population of blood donors in Wuhu city.Methods:A total of 210 RhD-samples from August 2021 to August 2022 were screened by serological test and collected from Wuhu Central Blood Station for the voluntary blood donor population.Exons 1 and 10 of the RHD gene were amplificated by PCR to determine whether the samples had the RHD gene.Exons 1-10 of the RHD gene were amplificated by PCR and zygosity analysis were performed in 82 samples containing D gene,and Sanger sequencing was performed on 55 samples containing all RHD exons to determine the genotype.Results:Among 210 RhD-specimens,128 cases(60.38％)had RHD gene deletion.27 cases had partial exons of RHD,including 2 cases with RHD*DVI.3/RHD*01N.01,24 cases with RHD*01N.04/RHD*01N.01,and 1 case with RHD-CE(2-10)/RHD*01N.01.55 cases had retained all of 10 exons,including 4 cases with RHD*01/RHD*01N.01,6 cases with RHD*15/RHD*01N.01,1 case with RHD*01W.72/RHD*01N.01,1 case with RHD*15/RHD*01EL.01,39 cases with RHD*01EL.01/RHD*01N.01,and the remaining 4 cases were determined to have no RHD gene deletion by zygosity analysis and sequencing showed the presence of 1227G＞A mutation loci.Conclusion:There is polymorphism in the molecular mechanism of RhD-D gene in Wuhu blood donor population,among which RHD*01EL.01 and RHD*15 are the main variants in this region.The results of this study provide a theoretical basis for RhD blood group identification and clinical blood transfusion in this region.
