Analysis of Frequencies and Subsets of Peripheral Helper T Cells in Patients with Immune Thrombocytopenia
10.19746/j.cnki.issn1009-2137.2024.05.033
- VernacularTitle:ITP患者外周血Tph细胞比例及亚群分析
- Author:
Wei-Ping LI
1
,
2
;
Zi-Ran BAI
;
Yu-Qin TIAN
;
Chun-Lai YIN
;
Xia LI
Author Information
1. 大连医科大学免疫教研室,辽宁大连 116044
2. 大连医科大学附属第二医院血液内科,辽宁大连 116027
- Keywords:
peripheral helper T cells;
immune thrombocytopenia;
CD4+T cells
- From:
Journal of Experimental Hematology
2024;32(5):1518-1519,1521-1523
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the frequencies and subset distribution of peripheral helper(Tph)T cells in patients with immune thrombocytopenia(ITP),and explore the pathogenesis of ITP.Methods:A total of 25 newly diagnosed ITP patients treated in The Second Affiliated Hospital of Dalian Medical University from January to December 2022 were selected,and 25 healthy volunteers(age-and sex-matched)were recruited as the control group.Flow cytometry was used to detect the subsets of CD4+T cells and Tph cells.Results:The frequency of effector memory(CCR7-CD45RO+CD4+)T cells in ITP patients was significantly higher than that in healthy controls(P＜0.05).The frequency of Tph cells in ITP patients was also significantly higher than that in healthy controls(P＜0.001),and most of the Tph cells in ITP patients were effector memory T cells.Furthermore,the expressions of T-cell costimulatory molecules in Tph cells,including ICOS and CD84,were similar to those in follicular helper T(Tfh)cells.CXCR3-CCR6-Tph(Tph2)subgroup was dominant in Tph cells,but the frequency of CXCR3+CCR6-Tph(Tph1)cells in ITP patients was much higher than that in healthy controls(P＜0.05).Conclusion:Tph cells,especially Tph1 cells,were abnormally expanded in ITP patients,which may be a potential etiology of ITP.
