Preparation Method and Quality Evaluation of Novel Frozen Human Platelets
10.19746/j.cnki.issn1009-2137.2024.04.044
- VernacularTitle:人新型冰冻血小板的制备方法与质量评价
- Author:
Yi-Zhe ZHENG
1
,
2
;
Dong-Dong LI
;
Geng-Wei YAN
;
Bao-Jian WANG
;
Ke WANG
;
Lei WANG
;
Shao-Duo YAN
;
Yan-Hong LI
;
Qiu-Xia FU
;
Zhen-Wei SUN
Author Information
1. 联勤保障部队第九八八医院,河南郑州 450042
2. 军事医学研究院卫生勤务与血液研究所,北京 100039
- Keywords:
novel frozen platelets;
preparation technique;
DMSO;
centrifugation conditions
- From:
Journal of Experimental Hematology
2024;32(4):1264-1270
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To optimize the technical parameters related to the preparation of novel frozen human platelets and formulate corresponding protocol for its preparation.Methods:Novel frozen human platelets were prepared with O-type bagged platelet-rich plasma(PRP),the key technical parameters(DMSO addition,incubation time,centrifugation conditions,etc.)of the preparation process were optimized,and the quality of the frozen platelets was evaluated by routine blood tests,apoptosis rate,platelet activation rate and surface protein expression level.Results:In the preparation protocol of novel frozen human platelets,the operation of centrifugation to remove supernatant was adjusted to before the procedure of platelets freezing,and the effect of centrifugation on platelets was minimal when the centrifugation condition was 800 xg for 8 min.In addition,platelets incubated with DMSO for 30 min before centrifugation exhibited better quality after freezing and thawing.The indexes of novel frozen human platelets prepared with this protocol remained stable after long-term cryopreservation.Conclusion:The preparation technique of novel frozen human platelets was established and the protocol was formulated.It was also confirmed that the quality of frozen platelets could be improved by incubating platelets with DMSO for 30 min and then centrifuging them at 800 ×g for 8 min in the preparation of novel frozen human platelets.
