Effect of Selinexor on Proliferation and Apoptosis of Acute Myeloid Leukemia Kasumi-1 Cells
10.19746/j.cnki.issn1009-2137.2024.04.017
- VernacularTitle:XPO1抑制剂塞利尼索对急性髓系白血病Kasumi-1细胞增殖和凋亡的影响
- Author:
Lu-Hui LIN
1
;
Sun-Qiao GAO
;
Xu-Qiao MEI
;
Da-Yi LIN
;
Yi-Feng CHEN
;
Su-Dan LIN
;
Li-Hong ZHUANG
;
Cong-Meng LIN
Author Information
1. 福建医科大学附属漳州市医院血液科,福建漳州 363000
- Keywords:
XPO1;
selinexor;
kasumi-1 cells;
proliferation;
apoptosis
- From:
Journal of Experimental Hematology
2024;32(4):1085-1090
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effects of selinexor,a inhibitor of nuclear export protein 1(XPO1)on the proliferation inhibition and apoptosis of Kasumi-1 cells in acute myeloid leukemia(AML).Methods:MTS method was used to detect the inhibitory effect of different concentrations of selinexor on the proliferation of Kasumi-1 cells at different time points.The apoptosis rate and cell cycle changes after treatment with different concentration of selinexor were detected by flow cytometry.Results:Selinexor inhibited the growth of Kasumi-1 cells at different time points in a concentration-dependent manner(r24 h=0.7592,r48 h=0.9456,and r72 h=0.9425).Selinexor inhibited Kasumi-1 cells growth in a time-dependent manner(r=0.9057 in 2.5 μmol/L group,r=0.9897 in 5 μmol/L group and r=0.9994 in 10 μmol/L group).Selinexor could induce apoptosis of Kasumi-1 cells in a dose-dependent manner(r=0.9732),and the apoptosis of Kasumi-1 cells was more obvious with the increase of drug concentration.The proportion of G0/G1 phase was significantly increased and the proportion of S phase was significantly decreased after the treatment of Kasumi-1 cells by selinexor.With the increase of drug concentration,the proportion of Kasumi-1 cells cycle arrest in G0/G1 phase was increased and the cell synthesis was decreased.Conclusion:Selinexor can promote the death of tumor cells by inhibiting Kasumi-1 cells proliferation,inducing apoptosis and blocking cell cycle.
