Pedigree Analysis of Hereditary Coagulation Factor Ⅻ Deficiency Caused by Compound Heterozygous Mutation p.Gly175Cys and p.Gly542Ser of F12 Gene
10.19746/j.cnki.issn1009-2137.2024.03.033
- VernacularTitle:F12基因p.Gly175Cys和p.Gly542Ser复合杂合突变导致的遗传性凝血因子Ⅻ缺陷症的家系分析
- Author:
Xiao-Li CHENG
1
;
Ting YANG
;
Liu YANG
;
Yi-Juan XIN
;
Mu HE
;
Lin ZHU
;
Jia-Yun LIU
Author Information
1. 空军军医大学第一附属医院检验科,陕西西安 710032
- Keywords:
blood coagulation factor Ⅻ;
missense mutation;
pedigree;
hereditary coagulation FⅫ deficiency
- From:
Journal of Experimental Hematology
2024;32(3):862-867
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To analyze the clinical phenotype and gene mutation of a genetic coagulation factor Ⅻ(FⅫ)deficiency pedigree and explore the molecular pathogenesis.Methods:The activated partial thromboplastin time(APTT)and FⅫ activity(FⅫ:C)were detected by clotting method.The FⅫ antigen(FⅫ:Ag)was tested with ELISA.All exons and flanks of F12 gene were determined by Sanger sequencing.ClustalX-2.1-win,PROVEAN and Swiss-Pdb Viewer software were used to analyze the conservatism of amino acids at the mutant site,forecast whether the mutant amino acids were harmful and confirm the influence of the mutation on protein structure.Results:The APTT of the proband prolonged to 71.3 s.The FⅫ:C and FⅫ;Ag were decreased to 5％and 6％,respectively.There were two heterozygous missense mutations c.580G＞T and c.1681G＞A detected in exon 7 and exon 14 of F12 gene,resultingin p.Gly175Cys and p.Gly542Ser,severally.Proband's father carried the p.Gly175Cys heterozygous mutation,while mother,brother and daughter had the p.Gly542Ser heterozygous mutation.Software analysis showed that both Gly175 and Gly542 were conserved,the two mutations were harmful and when mutations had occurred,the corresponding sites affected the protein local structure.Conclusion:The p.Gly175Cys and p.Gly542Ser compound heterozygous mutations are the molecular pathogenesis of the hereditary coagulation FⅫ deficiency pedigree.The p.Gly175Cys mutation has been detected for the first time in the world.
