Screening and Identification of lncRNA Related to Adipocity of Bone Marrow Microenvironment in Aplastic Anemia
10.19746/j.cnki.issn1009-2137.2024.02.043
- VernacularTitle:再生障碍性贫血骨髓微环境脂肪化相关lncRNA的筛选与鉴定
- Author:
Lei LIU
1
;
Huan-Huan ZHANG
;
Xian-Ning ZHANG
;
Lu-Lu LIU
;
Ming-Tai CHEN
Author Information
1. 济宁医学院附属医院血液科,山东济宁 272000
- Keywords:
aplastic anemia;
bone marrow mesenchymal stem cell;
PPARγ;
C/EBPα;
long noncoding RNA
- From:
Journal of Experimental Hematology
2024;32(2):610-616
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To systematically screen and identify long noncoding RNA(lncRNA)associated with bone marrow adiposity changes in aplastic anemia(AA).Methods:The PPARγ and C/EBPα ChIP-Seq data in ChIPBase was analyzed by bioinformatics and the potential lncRNA co-transcriptionally regulated by PPARγ and C/EBPα was screened.The expression of candidate lncRNA was verified by qRT-PCR in the in vitro adipogenic differentiation model of BM-MSC,BM-MSC infected with lenti-shPPARγ and lenti-shC/EBPα as well as clinical BM-MSC samples derived from AA and controls.Results:PPARγ and C/EBPα were significantly highly expressed in AA BM-MSC,and knock-down of PPARγ and C/EBPα impaired the adipogenic capacity of AA BM-MSC.PPARγ and C/EBPα cotranscriptionally activate LINC01230 promoter activity in binding sites dependant manner.The LINC01230 was also aberrantly highly expressed in AA BM-MSC compared with controls.Conclusion:PPARγ and C/EBPα are aberrantly expressed in AA BM-MSC and may promote the adipogenic differentiation of AA BM-MSC,and to a certain extent mediate the bone marrow adiposity alteration by transcriptionally activating LINC01230 expression.
