Reversal Effect of NVP-BEZ235 on Doxorubicin-Resistance in Burkitt Lymphoma RAJI Cell Line
10.19746/j.cnki.issn1009-2137.2024.02.024
- VernacularTitle:NVP-BEZ235逆转伯基特淋巴瘤RAJI细胞阿霉素耐药的研究
- Author:
Chun-Tuan LI
1
;
Xiong-Peng ZHU
;
Shao-Xiong WANG
;
Qun-Yi PENG
;
Yan ZHENG
;
Sheng-Quan LIU
;
Xu-Dong LU
;
Yong-Shan WANG
;
Dan WENG
;
Dan WANG
Author Information
1. 福建医科大学附属泉州第一医院血液内科,福建泉州 362200
- Keywords:
NVP-BEZ235;
Burkitt lymphoma;
doxorubicin-resistance;
reversal effect
- From:
Journal of Experimental Hematology
2024;32(2):476-482
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the reversal effect of NVP-BEZ235 on doxorubicin resistance in Burkitt lymphoma RAJI cell line.Methods:The doxorubicin-resistant cell line was induced by treating RAJI cells with a concentration gradient of doxorubicin.The levels of Pgp,p-AKT,and p-mTOR in cells were detected by Western blot.Cell viability was detected by MTT assay.IC50 was computed by SPSS.Results:The doxorubicin-resistant Burkitt lymphoma cell line,RAJI/DOX,was established successfully.The expression of Pgp and the phosphorylation levels of AKT and mTOR in RAJI/DOX cell line were both higher than those in RAJI cell line.NVP-BEZ235 downregulated the phosphorylation levels of AKT and mTOR in RAJI/DOX cell line.NVP-BEZ235 inhibited the proliferation of RAJI/DOX cell line,and the effect was obvious when it was cooperated with doxorubicin.Conclusion:The constitutive activation of PI3K/AKT/mTOR pathway of RAJI/DOX cell line was more serious than RAJI cell line.NVP-BEZ235 reversed doxorubicin resistance of RAJI/DOX cell line by inhibiting the PI3K/AKT/mTOR signal pathway.
