Mechanism about LMP1 of EB Virus Promoting Plasma Blast Diffe-rentiation of DLBCL Cell via mTORC1
10.19746/j.cnki.issn1009-2137.2024.01.035
- VernacularTitle:mTORC1在EB病毒LMP1促进DLBCL细胞母细胞化的机制研究
- Author:
Jing-Jing GAO
1
;
Xiong-Peng ZHU
;
Ming-Quan WANG
;
Xing-Zhi LIN
;
Yan-Ling ZHUANG
;
Hong-Jun LIN
Author Information
1. 福建医科大学附属泉州第一医院输血科,福建泉州 362000
- Keywords:
EB virus;
LMP1;
mTORC1 pathway;
CD38;
DLBCL;
plasmablast differentiation
- From:
Journal of Experimental Hematology
2024;32(1):219-224
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate possible mechanism on protien LMP1 expressed by EBV inducing plasmablast differentiation of DLBCL cell via the mTORC1 pathway.Methods:The expression levels of LMP1 protein,CD38 and the phosphorylation levels of p70S6K in EBV+and EBV-DLBCL cell lines were detected by Western blot.Cell lines overexpressing LMP1 gene stablely were constructed and LMP1 gene was silenced by RNAi.The expression of LMP1 gene was verified by RT-qPCR.The expression levels of LMP1 and CD38 and the phosphorylation levels of p70S6K in each group were detected by Western blot.Results:Compared with EBV-DLBCL cells,the expression of LMP1 was detected on EBV+DLBCL cells(P=0.0008),EBV+DLBCL cells had higher phosphorylation levels of p70S6K(P=0.0072)and expression levels of CD38(P=0.0091).Compared with vector group,the cells of LMP1OE group had higher expression levels of LMP1 and CD38(P=0.0353;P＜0.0001),meanwhile molecular p70S6K was phosphorylated much more(P=0.0065);expression of LMP1 mRNA was verified(P＜0.0001).Compared with si-NC group,expression level of LMP1 protein(P=0.0129)was not detected and phosphorylated p70S6K disappeared of LMP1KO group(P=0.0228);meanwhile,expression of CD38 decreased,although there was no significant difference(P=0.2377).Conclusion:LMP1 promotes DLBCL cells plasmablast differentiation via activating mTORC1 signal pathway.
