Expression of MicroRNA-3162-3p in Different Clinical Stages of Children with Primary Immune Thrombocytopenia and Its Signifi-cance
10.19746/j.cnki.issn1009-2137.2024.01.033
- VernacularTitle:MicroRNA-3162-3p在儿童原发性免疫性血小板减少症不同临床分期中的表达及其意义
- Author:
Xiao-Yan HU
1
;
Rui HE
;
Le-Yuan MI
;
Jiao-Jiao YIN
;
Fei-Fei JIN
;
Sheng-Dong ZHU
Author Information
1. 甘肃省妇幼保健院小儿综合内科,甘肃兰州 730050
- Keywords:
microRNA-3162-3p;
primary immune thrombocytopenia;
peripheral blood mononuclear cells;
Th17/treg imbalance
- From:
Journal of Experimental Hematology
2024;32(1):208-213
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the expression of microRNA-3162-3p in different clinical stages of childhood primary immune thrombocytopenia(ITP)and its significance.Methods:Ninety-six children with ITP were enrolled and divided into new diagnosis group(n=40),persistent group(n=30)and chronic group(n=26)according to the course of disease.80 healthy children were selected as the control group.Peripheral blood mononuclear cells(PBMNC)of ITP children and healthy children were isolated and cultured,and the expression of microRNA-3162-3p in PBMNC of subjects was detected by real-time fluorescence quantitative PCR.The contents of IL-17,IL-23,IL-10 and TGF-β in PBMNC of subjects were determined by ELISA.The correlation between microRNA-3162-3p and platelet count,IL-17,IL-23,IL-10 and TGF-β was analyzed.Results:Compared with the control group,the expression of microRNA-3162-3p and IL-10 in PBMNC and platelet count of ITP children were significantly decreased(P＜0.05),while IL-17,IL-23 and TGF-β were significantly increased(P＜0.05).With the prolongation of the disease course,the expressions of microRNA-3162-3p and IL-10 in PBMNC and platelet count were significantly decreased(P＜0.05),while the expressions of IL-17,IL-23 and TGF-β were significantly increased(P＜0.05).The expression of microRNA-3162-3p in PBMNC was positively correlated with platelet count and IL-10(r=0.716,0.667),and negatively correlated with IL-17,IL-23,and TGF-β(r=-0.540,-0.641,-0.560).Conclusion:MicroRNA-3162-3p expression is significantly reduced in PBMNC of children with ITP,and is involved in the regulation of Th17/Treg imbalance,which can be used as a potential therapeutic target of ITP.
