Inhibitory Effect of Metformin and Arsenic Trioxide on KG1a Cell Proliferation

VernacularTitle:二甲双胍与三氧化二砷对KG1a细胞增殖的抑制作用
Author: Meng LIU ; Shu-Min GUI ; Ming-Ming FENG ; Hui LIU ; Xiao-Hui SI ; Xin-Qing NIU
Keywords: arsenic trioxide; metformin; acute myeloid leukemia; Caspase 8; P62
From: Journal of Experimental Hematology 2024;32(1):66-70
CountryChina
Language:Chinese
Abstract: Objective:To investigate the effect of metformin and arsenic trioxide on KG1a cells proliferation of acute myeloid leukemia and its possible mechanism.Methods:CCK-8 method was used to detect the killing effect of metformin,arsenic trioxide and combined application on KG1a cells.Annexin V-FITC/P1 Dual Stain Flow Cytometry was used to detect the effect of combined application on apoptosis of KG1 a cells.Western blot was used to detect the expression of intracellular apoptosis-,autophagy-related protein.Results:Metformin and arsenic trioxide alone or in combination could inhibit the proliferation of KG1 a cells and induce apoptosis of KG1 a cells,and the proliferation inhibition rate and apoptosis rate in the combined drug group were higher than those in the drug group alone(P＜0.05).The combination of drugs induced upregulation of Caspase 8 protein and P62 protein expression and was higher than that in the drug group alone(P＜0.05).Conclusion:Metformin can synergize with arsenic trioxide to kill KG1a cells,and its mechanism of action may be related to inducing apoptosis and enhancing autophagy.