Deciphering Hypoplastic Myelodysplastic Syndrome and Aplastic Anemia via In-Depth Analysis of Lymphocyte Subsets
10.19746/j.cnki.issn1009-2137.2023.04.030
- VernacularTitle:淋巴细胞亚群在鉴别低增生性骨髓增生异常综合征和再生障碍性贫血中的价值
- Author:
Hong-Fei WU
1
;
Shi-Chong WANG
;
Jin-Bo HUANG
;
Jia-Li HUO
;
Ying-Qi SHAO
;
Xiang REN
;
Xing-Xin LI
;
Min WANG
;
Neng NIE
;
Jing ZHANG
;
Peng JIN
;
Mei-Li GE
;
Yi-Zhou ZHENG
Author Information
1. 中国医学科学院血液病医院,北京协和医学院(中国医学科学院血液学研究所),实验血液学国家重点实验室,国家血液系统疾病临床医学研究中心,细胞生态海河实验室,天津300020
- Keywords:
aplastic anemia;
hypoplastic myelodysplastic syndrome;
lymphocyte subsets;
flow cytometry
- From:
Journal of Experimental Hematology
2023;31(4):1125-1132
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the difference of lymphocyte subsets in peripheral blood(PB)between aplastic anemia(AA)and hypoplastic myelodysplastic syndrome(hypo-MDS)patients,meanwhile to compare the clinical parameters obtained from PB and bone marrow(BM).Methods:The lymphocyte subsets in hypo-MDS(n=25)and AA(n=33)patients were investigated by flow cytometry.Meanwhile,the differences in PB cell counts,biochemical indicators,BM cell counts and abnormal chromosomes between the two groups were analyzed.Results:The percentage of CD8+T cells in A A group was significantly higher than that in hypo-MDS group(P=0.001),while the percentage of CD4+T cells and the CD4+/CD8+ratio in AA group were obviously lower than those in hypo-MDS group(P=0.015 and0.001,respectively).Furthermore,the proportion of CD4+andCD8+activated T(TA)cells,and memory Tregs in AA group was distinctly lower than those in hypo-MDS group(P=0.043,0.015 and 0.024,respectively).Nevertheless,the percentage of CD8+naive T(TN)cells in AA patients was remarkably higher(P=0.044).And hypo-MDS patients had declined lymphocyte counts(P=0.025),increased levels of total bilirubin(TBil),lactate dehydrogenase(LDH),vitamin B12 and proportion of BM blasts than AA patients(P=0.019,0.023,0.027 and 0.045,respectively).Conclusion:In this study it was confirmed that the percentages of CD4+and CD8+TA cells,memory Tregs and CD8+TN cells were significantly different between AA and hypo-MDS patients,which provide an essential basis for the identification of these two diseases.
