Changes in serine metabolism and inflammation in CD4+T cells of cows with ketosis
10.16303/j.cnki.1005-4545.2024.11.18
- VernacularTitle:酮病奶牛CD4+T细胞中丝氨酸代谢变化与炎症变化
- Author:
Jingjing WANG
1
;
Ming LI
;
Jianan WEN
;
Shuang WANG
;
Chuang XU
;
Wei YANG
;
Bing-Bing ZHANG
Author Information
1. 黑龙江八一农垦大学生命科学技术学院,黑龙江大庆 163319
- Keywords:
CD4+T cells;
serine metabolism;
inflammation;
ketosis;
dairy cow
- From:
Chinese Journal of Veterinary Science
2024;44(11):2445-2451
- CountryChina
- Language:Chinese
-
Abstract:
To investigate the expression of serine metabolism related genes and inflammatory relat-ed genes in CD4+T cells of healthy and cows with ketosis.Firstly,CD4+T cells in peripheral blood of dairy cows were isolated by magnetic bead sorting.Second,expression of serine metabolism re-lated genes PHGDH,PAST1,PSPH,SHMT1,SHMT2,SDS,SFXN1 and inflammation related genes IL-6,IFN-γ,IL-17A,FOXP3,IL-10,TGF-β in CD4+T cells in healthy and ketosis cows was detected by fluorescence quantitative PCR.The transcription levels of PHGDH,PAST1 and PSPH in CD4+T cells of ketosis cows were significantly increased compared with healthy cows(P＜0.01),the transcription level and translation level of SDS were significantly increased(P＜0.01).The transcription levels of SHMT2 were significantly decreased(P＜0.05).Transcription and translation levels of SFXN1 were significantly decreased(P＜0.05).The transcription level of SHMT1 was decreased but not significantly.Compared with healthy cows,the transcription levels of IL-6,IFN-γ and IL-17A in CD4+T cells of ketosis cows were significantly increased(P＜0.01),the transcription levels of IL-10,TGF-β and FOXP3 were significantly decreased(P＜0.05).The results showed that the expression of genes related to serine synthesis increased,the expression of genes related to serine decomposition decreased,the expression of pro-inflammatory factors increased,and the expression of anti-inflammatory factors decreased,suggesting that serine metab-olism plays an important role in the inflammatory process of dairy cows.
