Construction of a Salmonella enterica eutR gene-deficient strain and characteriza-tion of some of its biological properties
10.16303/j.cnki.1005-4545.2024.11.15
- VernacularTitle:肠炎沙门菌eutR基因缺失株的构建及其部分生物学特性的鉴定
- Author:
Guixin ZHAO
1
;
Wenlong DUAN
;
Fengjie WANG
;
Xiaoyu ZHANG
;
Wan LIU
;
Lu ZHANG
;
Yu-Bin CHEN
;
Qiumei SHI
;
Tonglei WU
Author Information
1. 河北科技师范学院河北省预防兽医学重点实验室,河北秦皇岛 066004
- Keywords:
Salmonella enteritidis;
eutR gene;
biological characterization;
virulence factors
- From:
Chinese Journal of Veterinary Science
2024;44(11):2418-2423
- CountryChina
- Language:Chinese
-
Abstract:
The eutR gene deletion mutant of Salmonella enteritidis was successfully constructed by homologous recombination.Through the study of its biochemical characteristics,motility,resist-ance to stress in vitro and survival ability in RAW 264.7 cells,it was found that the biochemical characteristics and motility of the eutR gene deletion mutant of Salmonella enteritidis had no sig-nificant change compared with the wild type of Salmonella enteritidis.The ability of eutR gene de-letion strain of Salmonella enteritidis to resist acid,alkali and oxidation was significantly reduced,while the ability to resist heat was not significantly changed;the survival ability of eutR gene dele-tion strain in RAW 264.7 cells was significantly reduced compared with the wild type.In order to further analyze the effect of eutR gene on the expression of virulence factors of Salmonella enterit-idis,the relative expression levels of invH,ssav,ssrA,xthA,orf245,sodC,lrp,mrr1 and hflk virulence genes of the deletion strain and the wild strain were detected by SYBR Green PCR.It was found that the expression of the virulence factors mentioned above in the eutR gene deletion strain of Salmonella enteritidis was significantly down-regulated compared with that in the wild-type strain.The LD50 of the eutR gene-deleted strain of Salmonella enteritidis was determined by ani-mal experiments,and the results showed that the LD50 of the eutR gene-deleted strain was higher than that of the wild-type strain,indicating that the eutR gene could affect the virulence of Salmonella.This study clarified the effect of eutR gene on the survival ability,some biological characteristics and virulence of Salmonella enteritidis in macrophages,and provided a new gene knockout target for the development of attenuated Salmonella enteritidis genetic engineering vac-cine.
