Identification of parrot roundworms and analysis of its mitochondrial genome
10.16303/j.cnki.1005-4545.2024.11.10
- VernacularTitle:鹦鹉蛔虫的鉴定及其线粒体基因组的分析
- Author:
Na LI
1
,
2
;
Zhaowen REN
;
Yani WANG
;
Pian ZHANG
;
Tongbao SUN
;
Ziguo YUAN
;
Xi-Aohu WANG
Author Information
1. 华南农业大学兽医学院,广东 广州 510640
2. 广东省农业科学院动物卫生研究所广东省畜禽疫病防治研究重点实验室,广东 广州 510640
- Keywords:
parasitic roundworm;
mitochondrial genes;
phylogenetic analysis
- From:
Chinese Journal of Veterinary Science
2024;44(11):2379-2385
- CountryChina
- Language:Chinese
-
Abstract:
To identify the intestinal roundworms of Eclectus roratus and Psittacula alexandri from the Guangdong region by morphological analysis and molecular biology techniques,and to amplify the mitochondrial genome of parrot roundworms using PCR,as well as to carry out phylogenetic analyses based on the sequences of their mitochondrial genes.The results showed that the parrot roundworms identified in this identification belongs to Ascaridia.nymphii and its mitochondrial genome consists of 12 protein coding genes,22 tRNA genes,2 ribosomal RNA genes and two non-coding regions.Phylogenetic analyses showed that A.nymphii identified in this study is located in the same evolutionary branch as the previously reported Ascaridia sp.,is closely related and con-stitutes a separate clade of avian ascarids with pigeon roundworms and chicken roundworms.This study provides important data for the classification,molecular epidemiology and population genetic evolution of parrot roundworms.
