Whole-genome sequence analysis of Anhui strain of enzootic nasal tumor virus in goats
10.16303/j.cnki.1005-4545.2024.11.05
- VernacularTitle:山羊地方性鼻内肿瘤病毒安徽株全基因组序列分析
- Author:
Lingxu LI
1
;
Zhen WANG
;
Wenqing GUO
;
Ziyan LIN
;
Guangjun CHANG
;
Dawei YAO
Author Information
1. 南京农业大学动物医学院,江苏南京 210095
- Keywords:
goat;
enzootic nasal tumor virus;
enzootic nasal adenocarcinoma;
whole-genome sequence
- From:
Chinese Journal of Veterinary Science
2024;44(11):2341-2347
- CountryChina
- Language:Chinese
-
Abstract:
This study aims to obtain two whole-genome sequences of enzootic nasal tumor virus of goats(ENTV-2)from Anhui Province and analyzed the genetic diversity of ENTV-2 gene.Nasal secretion samples and blood samples of six goats with enzootic nasal adenocarcinoma(ENA)were collected from a goat farm in Anhui Province.The total RNA was extracted by the TRIzol method.The DNA interference was removed by the two-step reverse transcription.The ENTV-2 was detec-ted by PCR.Then,two positive samples were selected and five pairs of primers were used to ampli-fy the whole-genome sequences of ENTV-2.After sequencing and splicing,two sequences were up-loaded to the database for comparative analysis with the sequences in the NCBI database.Finally,the genetic evolution tree was constructed.ENTV-2 was detected in the nasal secretion samples,but not in the blood of the six ENA goats.The ENTV-2 genes were approximately 7 400 bp in length,named ENTV-2AH1(DDBJ accession no.:LC762616)and ENTV-2AH2(DDBJ accession no.:LC762617),respectively.Two sequences showed 99.2％and 99.1％homology with the Fujian strain(ENTV-2FJ)and Guangxi strain(ENTV-2-DA0),respectively.They were in the same evo-lutionary branch.In this study,two whole-genome sequences of ENTV-2 were obtained in Anhui for the first time,which can help to further study the genetic diversity of ENTV-2 in China.
