NLRX1 inhibits NDV proliferation by promoting ROS secretion
10.16303/j.cnki.1005-4545.2024.11.03
- VernacularTitle:NLRX1通过促进ROS分泌抑制NDV增殖
- Author:
Yongheng FU
1
;
Jindou LI
;
Jiaxin DING
;
Zhuang DING
;
Xiaohong XU
Author Information
1. 吉林大学动物医学学院,吉林长春 130062
- Keywords:
Newcastle disease virus;
nucleotide-binding domain and leucine-rich-repeat-containing family member X1;
intracellular inducible nitric oxide synthase;
reactive oxygen species
- From:
Chinese Journal of Veterinary Science
2024;44(11):2324-2333
- CountryChina
- Language:Chinese
-
Abstract:
To investigate the role of the nucleotide-binding domain and leucine-rich-repeat-contai-ning family member X1(NLRX1)in the replication of Newcastle disease virus(NDV),the ex-pression level of NLRX1 was examined following infection of 10-day-old specific pathogen-free(SPF)chicks and HD11 cells with the NDV NA-1 strain.Additionally,the proliferation of NDV,the expression of pro-inflammatory cytokines in HD11 cells,and the oxidative stress status were e-valuated in the presence of either overexpressed or underexpressed NLRX1.The results demonstra-ted that NA-1 infection led to an increase in NLRX1 expression both in vivo and in vitro.Further-more,overexpression of NLRX1 inhibited viral proliferation,enhanced the expression of cellularIL-1β,IL-6,and IFN-β,without affecting levels of autophagy or apoptotic cells.However,NLRX1 elevation resulted in elevated mRNA levels of iNOS,Keap1,Nrf2,NQO1,and HO-1 within 24 h.In conclusion,NLRX1 suppresses NDV proliferation by promoting early ROS production.
