Reverse genetics systems for coronavirus:Development and applications
10.16303/j.cnki.1005-4545.2024.10.26
- VernacularTitle:冠状病毒的反向遗传技术及其应用进展
- Author:
Jiyuan LIANG
1
;
Yucan ZHANG
;
Ruifeng HOU
;
Yue ZHANG
;
Yonghao XIA
;
Jin YUAN
;
Shaopo ZU
;
Zhanyong WEI
Author Information
1. 河南农业大学动物医学院动物病原与生物安全教育部重点实验室,河南郑州 450000
- Keywords:
coronavirus;
reverse genetic technology;
infectious clone;
transformation-associated re-combination cloning technology in yeast
- From:
Chinese Journal of Veterinary Science
2024;44(10):2283-2293
- CountryChina
- Language:Chinese
-
Abstract:
Coronavirus infection can cause serious respiratory and digestive system diseases in hu-mans and animals.In recent years,the frequent outbreaks and newly outbreaks of coronavirus in-fection have threatened global public health and the development of livestock and poultry.Howev-er,the development of anti-coronavirus drugs and vaccines was restricted due to the insufficient understanding of the mechanisms of coronavirus pathogenesis and cross species transmission.Re-verse genetic manipulation technology is a powerful tool in virological research,which can be used for the study of pathogenesis mechanisms,replication mechanisms and function analysis of protein.It is also be used in the development of attenuated or gene labeled vaccines,and antiviral drugs.Due to the large genome and complex structure of the coronavirus,the reverse genetic manipulation technology of coronavirus has been lagging for a long time.With the continuous updating of molec-ular biology methods,various new construction strategies have emerged.This article focuses on the construction strategy of the reverse genetic operating system for coronavirus,as well as its applica-tion in virus transmission and pathogenic mechanisms,and development of vaccines,which will provide favorable tools for the prevention and control of the coronavirus infection.
