Chebulagic acid regulates LPS-induced inflammatory response in dairy cow endom-etrial tissue through MAPK/NF-κB pathway
10.16303/j.cnki.1005-4545.2024.10.22
- VernacularTitle:诃子鞣酸通过MAPK/NF-κB通路调控LPS诱导的奶牛子宫内膜组织炎症反应的作用
- Author:
Ying YANG
1
;
Xinyu LIU
;
Feifan ZHAO
;
Wenrui GUO
;
Ruifeng GAO
Author Information
1. 内蒙古农业大学兽医学院,内蒙古呼和浩特 010018
- Keywords:
cow endometritis;
cow endometrial tissue;
chebulagic acid;
lipopolysaccharide;
inflamma-tory factor;
signaling pathway
- From:
Chinese Journal of Veterinary Science
2024;44(10):2251-2259
- CountryChina
- Language:Chinese
-
Abstract:
To investigate the role of chebulagic acid(CA)in regulating lipopolysaccharide(LPS)-in-duced inflammatory response in endometrial tissue of dairy cows,and to provide new ideas for the treatment and new drug development of endometritis in dairy cows.The endometrial tissues of dairy cows were isolated and cultured in vitro,stimulated with 1 mg/L LPS for 1 h and then co-cultured with CA(12.5,25.0,50.0,100.0 mg/L).Then the endometrial tissues of different treat-ment groups were collected for experiments.The protein secretion and gene expression levels of TNF-α,IL-6 and IL-1β were detected by ELISA and real-time fluorescence quantitative PCR.HE staining was used to observe the degree of endometrial tissue damage.The expressions of high mobility protein 1(HMGB-1)and hyaluronidase binding protein 2(HABP-2)were detected by immunofluorescence.The phosphorylation levels of ERK,p65 and IκBα were detected by Western blot.The results showed that the protein secretion levels of TNF-α at 6,24 h and IL-6 at 6,12 and 24 h,and the gene expression levels of IL-1β and IL-6 at 6,9,and 12 h and TNF-α at 6 h were sig-nificantly down-regulated after CA treatment with the LPS-induced endometrial tissue inflammation response model of dairy cows.HE staining showed that compared with the LPS group,the LPS+CA group had some improvements,the degree of epithelial cell exfoliation was reduced,the struc-ture of glands and blood vessels was relatively complete,the degree of inflammatory cell infiltra-tion was reduced,and there was no obvious necrosis or hemorrhage.The expression of HMGB-1 and HABP-2 in LPS+CA group was also significantly down-regulated.The phosphorylation levels of ERK,IκBα and p65 in the LPS+CA group were significantly decreased.In conclusion,CA can reduce LPS-induced inflammation in the endometrial tissue of dairy cows by inhibiting the activa-tion of MAPK and NF-κB pathways and down-regulating the expression of inflammatory factors in the uterine tissue.It is concluded that CA may be a potential therapeutic agent for endometritis in dairy cows and deserves further research and development.
