Effects of TIGAR on oxidative stress in adipose tissue of ketotic cows
10.16303/j.cnki.1005-4545.2024.10.20
- VernacularTitle:TIGAR对酮病奶牛脂肪组织氧化应激的影响
- Author:
Zhuo WANG
1
;
Xinxin FANG
;
Hexiang LI
;
Qiushi XU
;
Chuang XU
Author Information
1. 黑龙江八一农垦大学动物科技学院,黑龙江大庆 163000
- Keywords:
ketosis in dairy cows;
adipocytes;
oxidative stress;
TIGAR
- From:
Chinese Journal of Veterinary Science
2024;44(10):2234-2242
- CountryChina
- Language:Chinese
-
Abstract:
This experiment was divided into two parts:in vivo and in vitro.In the in vivo experi-ment,10 healthy and 10 ketotic cows were selected,and adipose tissues were collected.ELISA re-sults showed that malonaldehyde(MDA)content and reactive oxygen species(ROS)activity were higher in adipose tissues of cows with clinical ketotic compared with healthy cows,and glutathione peroxidase(GPP)activity was higher than that of cows with clinical ketotic.Western blot results showed that compared with the healthy group,adipose tissue of the ketotic group showed a signifi-cant increase in the expression level of TP53 induces glycolysis and apoptosis factors(TIGAR)protein,a significant up-regulation in the expression level of Nrf2-HMOX1 signaling pathway pro-tein,and a significant increase in the expression level of oxidative stress protein in adipose tissue of the ketotic group.The expression level of TIGAR protein was significantly up-regulated,the pro-tein level of Nrf2-HMOX1 signaling pathway was significantly up-regulated,and the protein levels of oxidative stress-related indexes SOD1,catalase(CAT)and glutathione S-transferase(GST)were significantly increased.It indicated that oxidative stress occurred in the adipose tissue of ketotic cows in vivo.In vitro experiments were carried out to detect the effect of TIGAR on oxida-tive stress in bovine adipocytes by adenoviral silencing or overexpression of TIGAR in isolated and cultured bovine primary adipocytes as well as by the addition of hydrogen peroxide in vitro for 2 h.The Western blot results showed that the hydrogen peroxide group showed enhanced oxidative stress compared with the control group,and the nuclear correlation factor 2 was significantly in-creased.correlation factor 2(Nrf2)pathway,decreased expression of Nrf2 and hemeoxygenase-1(HMOX1),and decreased expression of related oxidative stress proteins in the hydrogen peroxide group;compared with the hydrogen peroxide group,the protein expression levels of Nrf2-HMOX1 and related oxidative stress proteins were up-regulated in the group with overexpression of TIGAR and hydrogen peroxide,and the expression levels of related oxidative stress proteins were up-regu-lated in the group with overexpression of TIGAR and hydrogen peroxide.Expression level was up-regulated,and the expression of related oxidative stress proteins SOD1,CAT and GST increased.This indicates that overexpression of TIGAR alleviated hydrogen peroxide-induced oxidative stress in adipocytes.Protein expression of Nrf2-HMOX1 signaling pathway and oxidative stress-related proteins SOD1,CAT and GST were down-regulated in the silencing TIGAR plus hydrogen perox-ide group compared to the hydrogen peroxide group.It indicates that silencing TIGAR exacerbated the oxidative stress caused by hydrogen peroxide to adipocytes.
