Radix isatidis polysaccharide suppresses PRRSV replication through the TLR3/TRIF pathway

VernacularTitle:板蓝根多糖通过TLR3/TRIF通路抑制PRRSV复制
Author: Wenyi WU ¹ ; Xueyan HU ; Yuntian ZHANG ; Zhilong ZHANG ; Qiannan LI ; Yue JIN ; Mingfan YANG ; Hongying ZHANG
Author Information

1. 河南农业大学动物医学院,河南郑州 450002
Keywords: IRPS; PRRSV; TLR3; type Ⅰ interferon
From: Chinese Journal of Veterinary Science 2024;44(10):2197-2203
CountryChina
Language:Chinese
Abstract: The effect of Radix isatidis polysaccharide(IRPS)on TLR3/TRIF innate immune path-way and type Ⅰ interferon secretion in 3D4/21/CD163 cells infected by porcine reproductive and re-spiratory syndrome virus(PRRSV)was tested by Western blot and ELISA;moreover,the effect of IRPS on the immunosuppression infected by PRRSV was further verified with the TLR3 agonist poly(I∶C).The results showed that the protein levels of TLR3,TRIF,IRF3,IRF7 and type Ⅰ in-terferon secretion were significantly decreased at 18,24 h of PRRSV infection,while IRPS signifi-cantly inhibited this process;poly(I∶C)alleviated the protein levels of TRIF,IRF3 and IRF7 as well as the phosphorylation levels of IRF3 and IRF7 infected by PRRSV;at the same time,IRPS is synergistic with poly(I∶C).The results indicate that IRPS is able to alleviate immunosuppression caused by PRRSV infection via the TLR3/TRIF pathway.