Effects of CoCl2 on hypoxia-associated protein,lipid metabolism enzyme and insu-lin signaling pathway in primary bovine adipocytes
10.16303/j.cnki.1005-4545.2024.10.15
- VernacularTitle:氯化钴对原代牛脂肪细胞缺氧相关蛋白及胰岛素信号通路的影响
- Author:
Tong YANG
1
;
Yunhui FAN
;
Xidan ZHENG
;
Lu LU
;
Zhuo WANG
;
Qing LI
;
Cheng YANG
;
Chuang XU
;
Qiushi XU
;
Yuanyuan CHEN
Author Information
1. 黑龙江八一农垦大学动物科技学院,黑龙江大庆 163000
- Keywords:
hypoxia;
hypoxia inducible factor;
primary bovine adipocytes;
lipolysis
- From:
Chinese Journal of Veterinary Science
2024;44(10):2190-2196
- CountryChina
- Language:Chinese
-
Abstract:
This study utilized the CCK-8 assay to examine the effects of various concentrations of CoCl2(0,50,100,200,300,400 μmol/L)and different treatment durations(0,6,12,24,48 h)on the viability of adipocytes,in order to determine the most suitable treatment conditions.Western blot analysis was employed to investigate the impact of different concentrations of CoCl2(0,50,100,200,400 μmol/L)on the expression of hypoxia and its downstream key proteins in adipocytes.The results indicated that higher concentrations of CoCl2 led to lower adipocyte viability,with sig-nificant decreases in cell viability observed in the 300,400 μmol/L treatment groups(P＜0.01),while the 200 μmol/L group exhibited the highest cell viability.Compared to the control group,the 200 μmol/L CoCl2 treatment group showed a significant upregulation in the expression of hypoxia and its downstream signaling pathway key molecules:hypoxia-inducible factor 1-alpha(HIF-1α),glucose transporter type 4(GLUT4),vascular endothelial growth factor receptor 1(FLT-1),prolyl hydroxylase 2(PHD2),and vascular endothelial growth factor(VEGF)(P＜0.01).Addi-tionally,the 200 μmol/L CoCl2 treatment group exhibited higher levels of key lipolytic enzymes,including adipose triglyceride lipase(ATGL),perilipin 1(PLIN1),protein kinase A(PKA),and increased phosphorylation levels of hormone-sensitive lipase(HSL)in the 300 and 400 μmol/L groui ps(P＜0.01).CoCl2-mediated hypoxia in the 200 μmol/L treatment group also in-creased the protein expression of phosphatidylinositol 3-kinase(PI3K)and the phosphorylation level of protein kinase B(Akt).These findings suggest that adding 200 μmol/L CoCl2 can enhance the expression of hypoxia-related proteins,lipolytic enzymes,and insulin-related signaling proteins in primary bovine adipocytes.
