Isolation and identification of rabbit-derived Lactobacillus reuteri and evaluation of its probiotic function
10.16303/j.cnki.1005-4545.2024.10.08
- VernacularTitle:兔源罗伊乳杆菌的分离鉴定及益生效果评价
- Author:
Yu CHENG
1
;
Kun XIE
;
Yanping JIANG
;
Wen CUI
;
Jiaxuan LI
;
Xiaona WANG
;
Xinyuan QIAO
;
Lijie TANG
;
Yijing LI
;
Li WANG
Author Information
1. 东北农业大学动物医学学院,黑龙江哈尔滨 150030
- Keywords:
rabbit source;
Lactobacillus reuteri;
isolation and identification;
probiotic properties
- From:
Chinese Journal of Veterinary Science
2024;44(10):2136-2144,2293
- CountryChina
- Language:Chinese
-
Abstract:
Lactic acid bacteria(LAB)were isolated and cultivated from the intestinal contents of rabbits by MRS-CaCO3 solid medium.Identification was achieved through morphological observa-tion,Gram staining,physiological and biochemical characterisation,16S rDNA sequence analysis,and ERIC-PCR analysis.Strains displaying typical Lactobacilli characteristics were exanimated for their biological characteristics,resistance properties,adherence capacity in vitro,colonization abili-ty in vivo,and safety profile.In this study,a total of four strains of Lactobacillus reuteri were iso-lated from rabbits,all of which exhibited typical biological characteristics of LAB.These strains demonstrated inhibitory effects on common pathogenic bacteria in the gastrointestinal tract,with the primary inhibitory substance being bacteriocin.Furthermore,they showed sensitivity to chlor-amphenicol,rifampicin,and erythromycin,and displayed a degree of tolerance to gastrointestinal conditions and high temperature.These stains were capable of successful colonization in rabbits with a higher degree of safety.This study lays a foundation for the development of LAB prepara-tions for the prevention and treatment of rabbit intestinal diseases.
