Construction and identification of bivalent chimeric virus-like particles of Newcas-tle disease virus,serotype 4 and serotype 8 avian adenovirus
10.16303/j.cnki.1005-4545.2024.10.01
- VernacularTitle:新城疫、血清4型和8型禽腺病毒病二联三价嵌合型病毒样颗粒的构建及鉴定
- Author:
Chunhong GUO
1
,
2
;
Jindou LI
;
Jiaxin DING
;
Jiaxuan FENG
;
Kainan CHEN
;
Feng JIANG
;
Zhuang DING
Author Information
1. 吉林大学动物医学学院人畜共患传染病重症诊治全国重点实验室,吉林 长春 130062
2. 吉林元和元生物工程有限公司,吉林 长春 130022
- Keywords:
Newcastle disease;
serotypes 4 and 8 avian adenovirus disease;
insect baculovirus expres-sion system;
chimeric virus-like particles
- From:
Chinese Journal of Veterinary Science
2024;44(10):2087-2093,2115
- CountryChina
- Language:Chinese
-
Abstract:
A bivalent chimeric virus-like particle(ND-FAdV-4/8a/8b cVLPs)displaying NDV HN protein,FAdV4 fiber-2 protein,FAdV-8a Fiber protein and FAdV-8b Fiber protein was construc-ted based on insect baculovirus expression system and Newcastle disease virus-like particle vector platform and was identified using indirect immunofluorescence,Western blot and transmission e-lectron microscopy.The results showed that recombinant baculoviruses rBV-c8aFiber and rBV-c8bFiber expressing FAdV-8a Fiber and FAdV-8b Fiber were successfully constructed.In addition,all components of ND-FAdV-4/8a/8b cVLPs were correctly expressed,and ND-FAdV-4/8a/8b cVLPs was a nanoparticle about 100 nm in size,with a capsule membrane and fibers,providing a green and safe virus-like particle vaccine candidate for the control of Newcastle disease and avian adenovirus disease.
