Effect of porcine GHRH(1-44)expression plasmid on mouse growth and per-formance of pregnant sows
10.16303/j.cnki.1005-4545.2024.09.28
- VernacularTitle:猪GHRH(1～44)表达质粒对小鼠生长以及妊娠母猪生产性能的影响
- Author:
Yukai WEI
1
;
Yun WANG
;
Yuxuan WANG
;
Qianyun XI
;
Jiajie SUN
;
Ting CHEN
;
Junyi LUO
;
Hequn SHI
;
Yongliang ZHANG
Author Information
1. 华南农业大学动物科学学院广东省动物营养调控重点实验室/国家生猪种业工程中心/猪禽种业全国重点实验室,广东 广州 510642
- Keywords:
GHRH expression plasmid;
mice;
pregnancy sow;
GHRH
- From:
Chinese Journal of Veterinary Science
2024;44(9):2063-2071
- CountryChina
- Language:Chinese
-
Abstract:
The study adopted muscle injection of pVAX1-SP-GHRH(1-44)expression plasmid and electrostimulation to determine its effects on mouse growth and sow production performance.One hundred and fifty four-week-old C57 BL/6 female mice were randomly divided into 6 groups of 5 replicates each.Muscle single-injection followed by electrostimulation was performed.The con-trol group received an empty plasmid injection(80 μg/kg),while the treatment groups received pVAX1-SP-GHRH(1-44)plasmid injections(20,40,80,120,160 μg/kg).Twenty healthy preg-nant sows were randomly divided into 2 groups,each with 10 sows.Electrostimulation treatment was applied to the semimembranosus muscle of the pregnant sows after a single injection.The con-trol group received physiological saline injection,while the plasmid group received a 2 mg pVAX1-SP-GHRH(1-44)expression plasmid injection.Mouse weight,feed intake,and serum GHRH and IGF-1 levels were measured at days 0,7,14,21,and 28 after injection.Pregnant sows were bled via the tail vein at days 0,14,28,and 42 after injection,and their serum was separated to measure serum GHRH and IGF-1 levels.The birth weight,placental weight,number of piglets born,number of healthy piglets,number of weak piglets,number of deformed piglets,number of stillborn piglets,and number of mummified piglets were recorded at day 14.The mouse study re-sults showed that muscle injection of pVAX1-SP-GHRH(1-44)plasmid followed by electrostim-ulation could promote mouse feeding and increase weight gain(P＜0.05),significantly increase mouse serum GHRH and IGF-1 levels(P＜0.05),and maintain its effects until day 21.The results of the pregnant sow study showed that the average birth weight of the piglets in the plasmid group was significantly increased(P＜0.01),and the placenta weight was significantly increased(P＜0.05).The serum GHRH and IGF-1 concentrations in the plasmid group sows were significantly increased(P＜0.01).The study results showed that muscle injection of pVAX1-SP-GHRH(1-44)expression plasmid followed by electrostimulation could promote mouse feeding and increase weight gain,and also significantly improve the average birth weight and placental weight of the piglets in pregnant sows.
